Chemical Proteomics 2017, 1 ECTS credit
National course open for PhD students (prioritized), postdocs, researchers and other employees in need of skills of proteome-wide proteomics with aims in drug discovery and development, from all Swedish universities.
Responsible teachers: Massimiliano Gaetani, Roman Zubarev
Lectures will be also given by Mikhail Savitski, Janne Lehtiö and Rozbeh Jafari
If you don’t receive information according to the time period above, contact firstname.lastname@example.org.
A course fee of 1300 SEK will be invoiced to accepted participants (includes the coffee, lunches and course dinner). NOTE – SciLifeLab cannot invoice individuals.
This course will cover most updated chemical proteomics strategies available for identifying proteome-wide compound-target interactions, which ChemProt provides at the state-of-the-art level.
Topics will include:
- Introduction on mass spectrometry-based proteomics approaches used to identify drug/compound target molecules, by using protein extracts and cultured cells.
- Thermal protein profiling (TPP): how to benefit from ligand inducted changes of protein thermal stability to probe potential interactions using entire cells and protein extracts.
- Functional identification of target by expression proteomics (FITExP): how to benefit directly from quantitative, most relevant and specific responses of cell proteome to drug treatment for the identification of targets and potential keys of the drug´s efficacy.
- Hydrogen/Deuterium (H/D) exchange mass spectrometry (HDX MS): how to elucidate the interaction interface and map the binding site of a drug on a particular protein by H/D exchange and mass spectrometry.
- Knowledge of basic in vitro assays using cells for drug/compound testing
- Basic knowledge in experimental design and statistical methods
- Basic knowledge in proteomics analysis
- Practical interest into unbiased identification and analysis of the interaction between a drug/compound of interest and its protein target(s), and/or into unbiased discovery of the key proteins and their related mechanism of cell responses upon drug/compound treatment.
Due to space constraints, there is a maximum number of allowed participants. If we receive more applications, participants will be selected based on several criteria. Selection criteria include correct entry requirements, motivation to attend the course as well as gender and geographical balance.