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Christian Eggeling, December 8

SciLifeLab The Svedberg seminar series

Monday, December 8

Christian Eggeling

Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital,  Oxford, United Kingdom

C.E. holds a diploma and PhD in Physics from the University of Göttingen. During his PhD he optimized single-molecule detection in solution using different fluorescence spectroscopy techniques as well as investigating mechanisms of photobleaching and dark state transitions. From 2000 to 2003 he was a research scientist at Evotec, Hamburg, Germany, developing advanced fluorescence microscopy and spectroscopy techniques for drug high-throughput screening. End 2003 he moved to the department of Prof. S. Hell at the MPI of Biophysical Chemistry, Göttingen as a senior scientist, where he was involved in pushing the field of optical super-resolution microscopy, specifically the biological applicability of STED microscopy. Since end 2012 he is a principal investigator in the Human Immunology Unit and the scientific director of the Wolfson Imaging Centre at the Weatherall Institute of Molecular Medicine, University of Oxford, who recognized him with the title Professor of Molecular Immunology in 2014. In Oxford he is applying and further developing optical super-resolution microscopy for the investigation of subtle changes during the response of immune cells to infection.

Membrane bioactivity investigated by optical super-resolution STED(-FCS) microscopy

Plasma membrane interactions such as the transient protein-protein or protein-lipid complexes, the formation of lipid nanodomains (often denoted “rafts”), or diffusional restrictions by the cortical cytoskeleton are considered to play a functional part in a whole range of membrane-associated processes. However, the direct and non-invasive observation of such structures in living cells is impeded by the resolution limit of >200nm of a conventional far-field optical microscope. Here we present the use of the combination of super-resolution STED microscopy with fluorescence correlation spectroscopy (FCS) for the disclosure of complex nanoscopic dynamical processes. By performing FCS measurements in focal spots tuned to a diameter of down to 30 nm, we have obtained new details of molecular membrane dynamics, such as of transient lipid-protein interactions and of diffusional restrictions by the cortical cytoskeleton. Further insights will be given for molecular dynamics in the plasma membrane of immune cells, specifically during T-cell activation.


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