Extraction and quality control of samples for next-generation sequencing

Date: Tuesday 25 November 2014
Time: 9:00 – 11:00
Venue: Lunch Room, floor 2, Gamma building, SciLifeLab Stockholm

Next-generation sequencing (NGS) has led to a revolution in life science research and has driven breakthrough findings leading to a deeper understanding of genomes, transcriptomes and biological processes in health and disease.

Sample extraction and control over quality and quantity is a key aspect to succeed with a next-generation sequencing project. If you are interested to learn about how to extract, measure the concentration and assess the quality of your nucleic acids for successful generation of NGS libraries and how it can impact analysis – join NGI/SciLifeLab and Illumina for a seminar on sample extraction and validation!

During the seminar, you will learn about the wide array of Illumina library preparation kits andhow to quality control (QC) your samples for these kits. You will also get information about the sample reception routines at NGI/SciLifeLab and the sample requirements for different protocols, as well as activities on-going to validate analysis of different input types.

The event also offers an opportunity to speak one-on-one with Illumina and SciLifeLab specialists on how next generation sequencing can impact your work on DNA and RNA applications.

Please join us to get the most out of NGS technology!


9.00 – 9.05 Welcome  (Sverker Lundin and Pawel Zajac)

9.05 – 9.50 Preparation and QC of samples for the wide array of Illumina library prep

kits  (Pawel Zajac, Nordic Marketing Technical Specialist, Illumina)

9.50 – 10.10 NGI/SciLifeLab reception control  (Mattias Ormestad, Project Coordinator, NGI)

10.10 – 10.25 Coffee break

10.25 – 10.35 Input quantity and quality effects on mRNA sequencing results  (Phil Ewels,

Bioinformatician, NGI)

10.35 – 10.45 Evaluating the final sequencing library – a short introduction  (Pawel Zajac,

Nordic Marketing Technical Specialist, Illumina)

10.45 – 11.00 Concluding remarks  (Sverker Lundin and Pawel Zajac)


Please register via email: pzajac@illumina.com

Cannot attend, but wish to access the presentations? Please contact pzajac@illumina.com

If you would like a project meeting with NGI please contact genomics_support@scilifelab.se