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SciLifeLab seminar series-Campus Solna, Susana Cristobal – Cancelled


Tuesday, April 14, 2020 at 15:15 – Cancelled

Prof. Susana Cristobal

Department of Biomedical and Clinical Sciences, Cell Biology, Faculty of Medicine, Linköping University, Linköping, Sweden.

Title of the Talk: Environmental pollution in the One Health concept, proteomics-based solutions

The One Health initiative connects human and animal health with their shared environment. The One Health concept harmonizes with my mission as a scientist. My mission is to develop cost-effective methodologies for environmental and human health safety that could provide holistic information with high resolution, high sensitivity, multiple readout capabilities that integrated into predicting and modelling.  The society has targeted least 17 sustainable developmental for 2030 and many of them merged into the challenge of being exposure to environmental pollution. At Cristobal lab, we focus now on 3 challenges that proteomics-based method can elucidate: i) Deciphering the mechanism of actions of pollutants as bioactive compounds such as metabolism disrupting chemicals. One of the keys to understand the effects of a bioactive compound in a living cell is to predict the mechanisms of action. The initial step is an unbiased identification the molecular targets at the exposed cells. We have implemented a method, thermal proteome profiling that was developed to assess at the proteome level all possible drug targets[1]. We have modified it for its application to any bioactive compounds and it is named bioactive thermal proteome profiling (bTPP)[2]. ii)  Assess adverse effects of exposure to uncharacterized pollutant mixtures is one of the challenges of toxicology today. It is known that the effects from mixtures are not just an addition of effects from each compound of the mixture. Moreover, there is not yet any methodology that could offer a clear answer when neither the chemical structure is known, nor the concentration and ratio in the mixture could be measured. We propose the application of our method, bTPP, to address this problem and discuss the methodological opportunities. iii) Fate and effects of nano-sized environmental pollutants are complex because the dose does not make the poison. Moreover, the uptake processes, transfer of cellular barriers are based not only on the pollutant but also on the absorbed molecules. We developed a method, SUSTU[3], a surface proteomics method that can improve the understanding to the fate of nanoparticles or nanoplastics in an organism. Proteomics-based methods can offer to the One Health concept opportunities to explore scientific principles and develop methods from a different paradigm.

 

  1. Savitski MM, Reinhard FB, Franken H, Werner T, Savitski MF, Eberhard D, Martinez Molina D, Jafari R, Dovega RB, Klaeger S et al: Tracking cancer drugs in living cells by thermal profiling of the proteome. Science 2014, 346(6205):1255784.
  2. Carrasco Del Amor A, Freitas S, Urbatzka R, Fresnedo O, Cristobal S: Application of Bioactive Thermal Proteome Profiling to Decipher the Mechanism of Action of the Lipid Lowering 13(2)-Hydroxy-pheophytin Isolated from a Marine Cyanobacteria. Mar Drugs 2019, 17(6).
  3. Kuruvilla J, Farinha AP, Bayat N, Cristobal S: Surface proteomics on nanoparticles: a step to simplify the rapid prototyping of nanoparticles. Nanoscale Horizons 2017, 2(1):55-64.