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SciLifeLab The Svedberg seminar series 2014-04-28

Richard M. Caprioli

Department of Biochemistry, Chemistry, Pharmacology and Medicine, Director, Mass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, US

Dr. Caprioli’s general research interests lie in discovery of temporal and spatial processes in biological systems using mass spectrometry. This work has included technology developments in the areas of electrospray and laser desorption ionization mass spectrometry and their applications to intact tissues as well as other biological samples. He was named one of the “Pioneers in Proteomics” by the National Institutes of Health and has received several awards (the Thomson Medal Award from the International Mass Spectrometry, the Donald H. Coffey Award from the Society for Basic Urologic Research, the Field and Franklin Award from the American Chemical Society, the Eastern Analytical Society 2010 Award for Achievements in Mass Spectrometry and the HUPO Distinguished Achievement Award in Proteomic Sciences). Dr. Caprioli was elected a Fellow of the American Association for the Advancement of Science (AAAS) in November, 2012 and is currently serving a 3-year term on the Board of Directors of the HUPO and has been a member of the Board of Directors of the US HUPO since its inception.

Imaging Mass Spectrometry: Molecular Mapping Beyond the Microscope

MALDI Imaging Mass Spectrometry produces molecular maps of peptides, proteins, lipids and metabolites present in intact tissue sections. It employs direct laser irradiation to map the location of specific molecules from fresh frozen and formalin fixed tissue without the need of target specific reagents such as antibodies. Technological advances have produced images of high spatial resolution (1-10 microns) and at high speeds so that a typical sample tissue can be imaged in just a few minutes. Biocomputational approaches will be described including work on ‘image fusion’ in combining data attributes of both MS and microscopy. Applications include research in diabetic nephropathy in both a mouse model and in the human disease and also in the development of molecular signatures of skin lesions to differentiate benign lesions from metastatic melanoma. Imaging MS has been applied to drug and metabolism studies both in specific organs and also in intact whole animal sections.

Host: Per Andrén