SciLifeLab The Svedberg seminar series 2014-05-12


Charlie Boone

Banting and Best Department of Medical Research, The Donnelly Center for Cellular and Biomolecular Research, University of Toronto, Toronto, Canada

Dr. Charlie Boone is a principal investigator at the Terrence Donnelly Centre for Cellular and Biomolecular Research and a professor at the Banting & Best Department of Medical Research, Department of Molecular Genetics, University of Toronto. He is also a team leader at the, Chemical Genomics Research Group, RIKEN, Japan. His research focuses on mapping yeast genetic networks on a large scale in order to assign gene function.

Yeast Systems Biology: The Genetic Landscape of a Cell

Genetic interactions can play an important role in determining the relationship between genotype and phenotype and may underlie a significant component of the “missing heritability” in current genome-wide association (GWAS) studies. To explore the general principles of genetic networks, we’ve taken an unbiased and global approach to map digenic interactions in the budding yeast Saccharomyces cerevisiae. We developed Synthetic genetic array (SGA) analysis, which automates yeast genetics, enabling the combinatorial construction of defined mutants and the mapping of genetic interactions quantitatively. We are generating a comprehensive genetic landscape for yeast, examining networks derived from an analysis of all gene pairs, covering both nonessential and essential genes. Constructing a higher order network driven by genetic interaction profiles reveals the roles of specific genes and traces a global functional wiring diagram of the cell. Ultimately, our findings in yeast may allow us to explore genetic interactions underlying the heritability of traits in other organisms, including humans, and the design of synthetic lethal cancer therapies.

Host: Ylva Ivarsson