NEW WEBSITE We would like to invite you to a sneak peak of our new website before it goes live. Visit the new site

Structural Biology Discussion Group

Structural biology aims direct visualization of macromolecules and therefore provides the most definitive way to describe its mechanisms of action. High resolution cryo-electron microscopy (cryo-EM) is emerging and developing technique in structural biology capable of producing density maps for direct atomic modelling.

Every Thursday at 9.00, we gather a discussion group to better understand what is the current practice of cryo-EM, critically assess where it falls short, present the most recent developments and applications to interesting biological problems. We encourage everyone to take active part.

Location: SciLifeLab Stockholm, Alpha 5, Kinnekulle room.

Presenter: Jose Miguel de la Rosa Trevin
Home lab: Spanish National Research Council
Title: SCIPION workshop



Theoretical session:

9:00 – 10:30, Alpha-5, Kinnekulle room.

General introduction to cryo-EM single particle.


Practical session I:

10:45 – 13:00, Gamma-3, Amunts lab.

– Movies alignment (Xmipp, MotionCorr).

– CTF estimation and screening (Ctffind4).

– Particle picking (Xmipp, Eman).


13:00 – 14:00   Lunch break


Practical session II:

14:00 – 17:00, Gamma-3, Amunts lab.

– 2D classification (Spider).

– More picking with templates (Relion).

– 2D classification (Relion, Xmipp).

– Initial volume methods (Various packages).

– 3D classification and refinement (Relion).

For more details please contact Alexey Amunts.