Structural studies of chromatin complexes
Speaker: Song Tan, Department of Biochemistry & Molecular Biology, The Pensylvania State University
The packaging of eukaryotic DNA as chromatin affects all genetic processes, but we possess a poor understanding of how chromatin enzymes interact with the nucleosome unit of chromatin. My laboratory would like to understand how chromatin proteins and enzymes recognize and act on their nucleosome substrate. We have combined biochemical and structural approaches, including X-ray crystallography, to provide high resolution structural descriptions of the RCC1 chromatin protein and the Polycomb Repressive Complex 1 (PRC1) ubiquitylation enyzme in complex with the nucleosome. I will describe how we tackled structural characterization of these multicomponent complexes, and the paradigms for nucleosome recognition that have emerged from these studies.
This seminar is part of a seminar series hosted by SciLifeLab Fellows
Date: June 16
Venue: Air&Fire auditorium, SciLifeLab Solna
Host: Alexey Amunts