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Eukaryotic Single Cell Genomics

National facility

The Eukaryotic Single Cell Genomics facility aims at providing single cell transcriptomics and genomics services to the scientific community. Single cell analysis is an emerging technology that allows for the exploration of the transcriptome and genome content of individual cells. The facility offers streamlined single-cell RNA sequencing as well as whole genome DNA amplification to the scientific community in Sweden and beyond.

SERVICES

  • RNA-seq (full-length). Cell capture, lysis, reverse transcription and PCR, followed by library preparation and sequencing. Submit cells as a suspension, and obtain expression data in return. Up to 384 cells per day can be processed.
  • RNA-seq (quantitative 5′ tag). Cell capture, lysis, reverse transcription and PCR, followed by library preparation and sequencing. Submit cells as a suspension, and obtain expression data in return. Up to 384 cells per day can be processed.
  • Whole-genome amplification. Streamlined lysis and whole-genome amplification (WGA) of individual cells using multiple-strand displacement amplification (MDA). Up to 384 cells per day can be processed.

EQUIPMENT

  • Fluidigm C1 microfluidic processors.
  • Tecan Freedom EVO liquid handling robot.
  • Nikon inverted microscope.

APPLICATIONS

  • Study heterogeneity within putatively homogeneous cell populations
  • Unbiased discovery of cell types in complex tissues
  • Genome/exome sequencing or SNP analysis of rare cells such as circulating tumor cells.
  • Characterizing the cellular and genetic composition of tumors.