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Single Cell Proteomics

National facility

The Single Cell Proteomics Facility aims to provide access to state-of-the art methods to detect proteins, or protein combined with RNA, in single cells for the Swedish and international scientific community.  

The SciLifeLab Single Cell Proteomics facility and Olink Proteomics are excited to announce the launch of a beta testing initiative. We invite individuals from the Swedish academic scientific community to test a prototype single cell 92plex panel of proximity extension assays (PEA) targeting proteins involved in a number of cellular processes, including differentiation, proliferation and apoptosis. Accepted users are invited to test the PEA panel for free but there will be some associated costs with the testing. For more information, including the full list of available assays, terms and how to apply, please contact Caroline Gallant at caroline.gallant@igp.uu.se. Deadline to apply is June 23rd.
 

SERVICES

  • Cell isolation from single cell suspensions via flow sorting or using the Fluidigm C1 microfluidic system
  • 96-plex protein detection in single or a few human cells using proximity extension assays (PEA; Olink Biosciences)
  • Targeted protein analysis, alone or combined with targeted or global RNA analysis
  • High throughput qPCR via the Fluidigm BioMark HD system

EQUIPMENT

  • C1 Single-Cell Auto Prep system, Fluidigm
  • Realtime PCR readout via BioMark HD system, Fluidigm

APPLICATIONS

  • Investigate heterogeneity in cell populations or tissues at the level of protein +/- RNA
  • Validate or follow-up findings from transcriptomic studies
  • Investigate RNA-protein expression dynamics