Nicola Crosetto


Nicola Crosetto
Crosetto Lab for Quantitative Cancer Science and Technology Development
Karolinska Institutet

Research Interests

Our mission is to develop simple, quantitative, and cost-effective molecular technologies that can be applied to tackle fundamental questions in biology and biomedicine. Specifically, we are interested in developing means to accurately quantify genetic alterations, epigenetic modifications, and transcriptional programs at genome-wide scale and high spatial resolution in clinical tumor samples. In the long run, we aim at contributing to view cancers as complex ecosystems, and to incorporate multi-omic measurements of tumor diversity into clinical practice.

Group members

Nicola Crosetto, Principal Investigator (Oncology, Molecular Biology)
Silvano Garnerone, Project Leader (Physics)
Xiaolu Zhang, Postdoctoral Associate (Hematology, Molecular Biology)
Reza Mirzazadeh, PhD Student (Molecular Biology)

Key publications

 

1) Spatially resolved transcriptomics and beyond.
Crosetto N*, Bienko M, van Oudenaarden A.
Nat Rev Genet. 2015 Jan;16(1):57-66. PMID: 25446315
*Corresponding author

2) FuseFISH: robust detection of transcribed gene fusions in single cells.
Semrau S, Crosetto N*, Bienko M, Boni M, Bernasconi P, Chiarle R, van Oudenaarden A. Cell Rep. 2014 Jan 16;6(1):18-23. PMID: 24373969
*Equally contributing first author

3) Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing.
Crosetto N*, Mitra A, Silva MJ, Bienko M, Dojer N, Wang Q, Karaca E, Chiarle R, Skrzypczak M, Ginalski K, Pasero P, Rowicka M, Dikic I. Nat Methods. 2013 Apr;10(4):361-5. PMID: 23503052
*Corresponding author

4) A versatile genome-scale PCR-based pipeline for high-definition DNA FISH.
Bienko M, Crosetto N*, Teytelman L, Klemm S, Itzkovitz S, van Oudenaarden A.
Nat Methods. 2013 Feb;10(2):122-4. PMID: 23263692
*Equally contributing first author

Group homepage/external homepage

http://bienkocrosettolabs.org