Chemical Biology Consortium Sweden organizes a course that gives a practical basis for assay development in high throughput screening. The course is given as complement to the theoretical on-line course “Introduction to high throughput screening”. Passed examination of the theoretical course is a prerequisite for taking the practical.
Venue: Laboratories of Chemical Biology Umeå (LCBU), Umeå University, Umeå
Responsible teacher/s: Stina Berglund and Mikael Elofsson, Department of Chemistry, Umeå University
Register: by sending an e-mail to email@example.com. Include a letter with motivation to take the course. The number of participants is limited.
The course gives a practical basis for assay development in high throughput screening and is given as a practical complement to the course “Introduction to high throughput screening”. High throughput screening (HTS) is a technique used to identify biologically active small organic molecules through screening of compound libraries with the help of robust test systems and advanced instrumentation. The course includes several aspects of assay development applicable to both target-based and cell-based assays, however, the focus will be on cell-based assays. The assay carried out in the course includes cell culturing and bacterial infection, thus experience in cell culturing and good microbial lab practice is required. Parameters of importance for optimization of a cell based assay will be tested empirically in the laboratory and their significance calculated using Z’. During the course a small-scale screen will be executed. The students will thereby be given knowledge about the importance of good screening logistics and gain insight into the amount of data generated and handling thereof. During the course the students will be introduced to instrumentation commonly used in high throughput screening such as liquid handling instrumentation, plate readers and high content screening microscope.
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