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DTSTART;TZID=Europe/Stockholm:20221011T080000
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DTSTAMP:20260404T031646
CREATED:20220912T161617Z
LAST-MODIFIED:20220912T161619Z
UID:142889-1665475200-1665680400@www.scilifelab.se
SUMMARY:Cryo-EM sample preparation and data collection course
DESCRIPTION:The purpose of the course is to prepare and train Cryo-EM facility users in sample preparation methods\, introduce users to the image data acquisition workflow\, expand knowledge about cryo-EM methods among researchers and show that everyone can learn how to use cryo-EM for structure and cell biology. \n\n\n\nThe course is open for facility users or potential facility users\, such as PhD students\, postdocs\, and researchers within the life sciences who are curious and will profit from cryo-EM skills. Swedish and international course participants are welcome. To attend\, the course participants must have experience in basic electron microscopy and be familiar with structure or cell biology. \n\n\n\nThis course is supported by SciLifeLab\, CryoNET and Umeå University and organized by the SciLifeLab Cryo-EM facility\, both UmU and SU nodes together. \n\n\n\nApplication deadlineApplication should be submitted before September 16\, 2022 \n\n\n\nRead more\n\n\n\nCourse organizersCamilla Holmlund and Michael Hall \n\n\n\nLocationUCEM and Chemical Biological Centre (KBC) BuildingUmeå UniversityLinnaeus väg 6 \n\n\n\nTeachers and course instructors \n\n\n\nLinda SandbladMichael HallLars-Anders CarlsonCamilla HolmlundAdditional teachers t.b.a\n\n\n\nSocial programA social program depending on the current corona restriction will be communicated to the participants after acceptance. \n\n\n\nCourse feeA course fee of 800 SEK\, including coffee and lunches\, will be invoiced to accepted participants after acceptance. \n\n\n\nCourse contentThis course will cover practical aspects of cryo-EM sample preparation and data acquisition.Topics covered: \n\n\n\nBasic theory of vitreous water\, how to handle a cryo sample and consequencesPlunge freezing methods\, tools and cryo-transferSample and instrument requirements for single particle and tomography methodsImage data acquisition\, concept of low dose and the cryo-specific workflowIntroduction to automatic data acqui­sition software\, EPU\, TomographyIntroduction to cryo-EM image processing methods\n\n\n\nThe course consists of lectures\, cryo lab work\, demonstrations and practical exercises for all participants\, hands-on experiences of microscopy operation and discussions. A detailed schedule will be provided to accepted participant. \n\n\n\nCourse literatureHandouts and material online. \n\n\n\nEntry requirementsPriority will be given to researchers involved in project where the use of cryo-EM is needed\, which are to be briefly described upon application. \n\n\n\nThe course will be held at an advanced level\, assuming that participants have a master’s degree or equivalent in a technical life science discipline. It aims to be relevant for a broad research community\, also to participants not working in research environments using cryo-EM today. \n\n\n\nA maximum of 15 participants will be admitted. Selection criteria include correct entry requirements\, motivation to attend the course as well as gender balance. \n\n\n\nExamination Participation in all parts of the course\, lectures\, lab\, microscopy demonstrations and evaluation are mandatory. SciLifeLab and Umeå University will provide successful participants with a course certificate and a recommendation for 1 ECTS
URL:https://www.scilifelab.se/event/cryo-em-sample-preparation-and-data-collection-course/
CATEGORIES:Course
ORGANIZER;CN="SciLifeLab Ume%C3%A5":MAILTO:umea@scilifelab.se
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