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X-WR-CALDESC:Events for SciLifeLab
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DTSTART;TZID=Europe/Stockholm:20260505T090000
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DTSTAMP:20260531T121226
CREATED:20260319T113141Z
LAST-MODIFIED:20260319T113713Z
UID:196529-1777971600-1777975200@www.scilifelab.se
SUMMARY:Live-Cell Super-Resolution Microscopy: Technological Progress\, Hybrid Methodologies\, and Applications in Omics
DESCRIPTION:Liangyi Chen\, New Cornerstone Investigator\, Institute of Molecular Medicine\, National Biomedical Imaging Center\, Peking University\, China. \n\n\n\nHost: Jan Ellenberg\, SciLifeLab \n\n\n\nAbstract\n\n\n\nIn this talk\, I will present our decade-long efforts to advance state-of-the-art live-cell super-resolution microscopy. By introducing spatiotemporal Hessian regularization\, we developed Hessian Structured Illumination Microscopy (Hessian-SIM)\, which substantially reduces phototoxicity and enables real-time\, long-term imaging of dynamic structures such as mitochondrial cristae and insulin vesicle fusion pores in living cells. We next engineered a dual-modality fluorescence/label-free microscope for panoramic super-resolution imaging\, facilitating the discovery of novel organelles. Our 4Pi-SIM and 3D multiplane SIM (3D-MP-SIM) techniques deliver ~100-nm isotropic resolution while achieving a tenfold increase in volumetric imaging speed for live cells.  \n\n\n\nFurthermore\, we introduced a universal sparse deconvolution algorithm that overcomes the optical Rayleigh diffraction limit. When applied to structured illumination\, it enables 60-nm resolution at 564 Hz for sustained live-cell imaging and enhances resolution in multiphoton and light-sheet microscopy in vivo. Finally\, I will discuss our recent developments in a SIM-FLIM system and the integration of live-cell super-resolution SIM imaging with single-cell RNA sequencing to bridge nanoscale dynamics with transcriptomic profiles.
URL:https://www.scilifelab.se/event/live-cell-super-resolution-microscopy-technological-progress-hybrid-methodologies-and-applications-in-omics/
LOCATION:Air&Fire\, SciLifeLab Stockholm\, Tomtebodavägen 23A\, Solna\, Sweden
CATEGORIES:Event
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