We would like to invite you to a virtual Seminar on Thursday, Oct 8th at 15.00. This webinar will focus on Micro-C and MNase-based HiChIP in transcriptional control and Chromatin structure as well as outline the advantages of MNase compared to traditional restriction-enzyme-based Hi-C.
Micro-C: Using MNase for chromatin conformation capture at the nucleosome level
by Cory Padilla, Ph.D. (Scientific Affairs at Dovetail)
The discovery of chromatin capture methods, such as Hi-C, has enabled the study of chromatin conformation and its role in gene regulation. However widespread use of restriction enzymes for chromatin fragmentation limits assay functional resolution and ultimately the types of features that can be visualized in the contact matrix.
The use of Micrococcal nuclease (MNase) to digest chromatin in a Hi-C assay, termed Micro-C, overcomes this barrier by enriching for uniform nucleosome-sized fragments. The resulting data increases assay performance and efficiency, enabling detection of and reducing the sequencing burden required for the detection of higher resolution and existing chromatin features respectively.
In our webinar, we will discuss Micro-C’s ability to capture, Enhancer-Promoter, Promoter-Promoter, and loop extrusion features that are missed with standard Hi-C approaches. By preserving the large-scale chromatin features such as A/B compartments, TADs, and CTCF mediated loops while enabling the description of chromatin at sub-TAD scales, Micro-C provides, a new ultra-resolution view of chromatin biology emerges.
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