New Approaches in Cell Based Assays

New Approaches in Cell Based Assays – Getting More Out Of Less?

Location: SciLifeLab, Tomtebodavägen 23A, Solna,

Seminar room Air and Fire in Gamma 2

SCIENTIFIC SEMINARS: 09:00 – 09.05

Welcome

09:05 – 09.20

Drug discovery in academia – Making more with less?

Bo Lundgren, Biochemical and Cellular Screening, SciLifeLab, Stockholm university

09.20 – 10.00

Application of the cellular thermal shift assay in microplate format

Thomas Lundbäck, Chemical Biology Consortium Sweden, SciLifeLab, Karolinska Institutet

10.00 – 10.40

Orthogonal approaches for drug discovery using the NEW plate reader EnSight from PerkinElmer

Carlo Zaniol, Application Specialist, PerkinElmer

10:40 – 11.10

COFFEE

11.10 – 11.50

Possibilities and pitfalls in large-scale image-based screening

Carolina Wählby Centre for Image Analysis, Division of Visual Information and Interaction at the Department of Information Technology, Uppsala University

11.50 – 12.30

High Content Imaging; a fully integrated and bioinformatics workflow

James Hutt, Application Specialist, PerkinElmer

Abstracts in PDF

WORKSHOP

Get more relevant results with a truly orthogonal approach – interrogate the cells from different angles to get more and true answers

In this workshop we demonstrate the capabilities of the EnSight™ Multimode Plate Reader by showing how to combine imaging with classical endpoint measurements. The well-imaging module provides image-based cytometry capabilities and includes brightfield, digital phase and up to 4 color fluorescent imaging. This enables the determination of inter-well and intra-well cell distribution, investigation of changes in cell morphology, and detection of fluorescent dyes, reporter genes or cell markers. This uncomplicated and seamless system allows the generation of more reliable cell-based assay data for many types of applications. The orthogonal approach will in short time generate a respectable amount of data, which can easily be visualized and correlated by the SpotFire software.

You will learn about the following technologies: brightfield, digital phase imaging (DPI), two colors fluorescence imaging, AlphaLISA detection of biomarkers, viability (ATP) and SpotFire analysis

Program in PDF

To register or if you have questions, please email Jannicke.kolmskog@perkinelmer.com at the latest on the 25th of May. Also inform if you wish to attend the workshop and which time slot (max 6 people/group). The numbers of seats are limited for the seminar. For the coffeebreak, let us know if you have any allergies.