Speaker: Joerg Bewersdorf, Department of Cell Biology and Department of Biomedical Engineering, Yale University
Optical Nanoscopy (super-resolution) techniques such as STED and FPALM/PALM/STORM (single-molecule switching, SMS) microscopy utilize either targeted or stochastic switching of fluorescent molecules to achieve ~25 nm spatial resolution – about 10-fold below the diffraction limit.  The last years have seen many improvements that make these technologies suitable for a rapidly expanding range of applications.
I will report about two recent breakthroughs that significantly expand the live-cell and 3D imaging capabilities of these techniques:
I will present the scientific foundation, technical realization and application of these new techniques.
Joerg Bewersdorf has financial interest in Hamamatsu and Bruker.
 T.J. Gould, S.T. Hess, and J. Bewersdorf “Optical Nanoscopy: from Acquisition to Analysis”, Annu. Rev. Biomed. Eng. 2012
 F. Bottanelli, E.B. Kromann, E.S. Allgeyer, R.S. Erdmann, S. Wood Baguley, G. Sirinakis, A. Schepartz, D. Baddeley, D.K. Toomre, J.E. Rothman, J. Bewersdorf “Two-color live-cell nanoscale imaging of intracellular targets”, Nature Communications 2016
 F. Huang, G. Sirinakis, E.S. Allgeyer, L.K. Schroeder, W.C. Duim, E.B. Kromann, T. Phan, F.E. Rivera-Molina, J.R. Myers, I. Irnov, M. Lessard, Y. Zhang, M.A. Handel, C. Jacobs-Wagner, C.P. Lusk, J.E. Rothman, D.K. Toomre, M.J. Booth, J. Bewersdorf “Ultra-high resolution 3D imaging of whole cells”, Cell 2016
This seminar is part of a seminar series hosted by SciLifeLab Fellows
Date: Nov 7
Venue: Air&Fire auditorium, SciLifeLab Solna
Host: Ilaria Testa