Date: 11/1 2021 to 27/1 2021 (5 days on site at Linköping University)
National course. The course is open for current and potential facility users such as PhD students, postdocs, group leaders and core facility staff in need of mass cytometry skills within all Swedish universities.
Application open: 1/9 2021
Application deadline: 1/12 2020
Confirmation to accepted participants: Yes
Responsible teachers: Jörgen Adolfsson (head of SciLifeLab Mass Cytometry facility at Linköping University)
Examinator: Marie larsson
Contact person: Jörgen Adolfsson (head of SciLifeLab Mass Cytometry facility at Linköping University)
Flow cytometry is the method of choice when single cell multiplex analysis of protein receptors are to be examined with fluorochrome labelled antibodies. Unfortunately problems with spectral overlap occur from the different fluorochromes necessitating colour compensation. This will in practice restrict experiments to analysing only 12-16 proteins on a single cell level. A mass cytometer or Cytometry by Time-Of-Flight (CyTOF), however, has the capacity to analyze up to 40 proteins at a single cell level. This is made possible by utilizing rare earth metals conjugated to antibodies instead of fluorochromes. The different masses of the individual metals are easily detected by the time-of-flight mass spectrometer thus rendering the need for compensation unnecessary.
This course is an introduction to mass cytometry. The participants will be trained to compare mass cytometry and flow cytometry methods. Lectures will cover various aspects such as processing data for the analysis and an overview of available methods for the analysis.
For this course you are also required to have experience of multi-parameter flow cytometry. Priority will be given to those who have a planned and/or on-going mass cytometry project