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DTSTART;TZID=Europe/Stockholm:20250310T080000
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UID:177647-1741593600-1741971600@www.scilifelab.se
SUMMARY:Workshop for Cryo-electron Tomography
DESCRIPTION:Location: UCEM facility (EM-building) and Chemical Biological Centre (KBC) building\, Umeå University\, Linnaeus väg 6\, Umeå \n\n\n\nDeadline for registration: 13 January 2025 \n\n\n\nCryo-electron tomography (cryo-ET) is a powerful structural technique. Its power comes from its versatility to study biological systems in vitro or in situ\, i.e. within cells or tissue. It has the ability to reach uncharted regions in biological systems at high resolution. Advancements in microscopes\, cameras\, and computation make it now possible to determine sub-10 Å 3-dimensional structures of molecules directly within cells. \n\n\n\nThis four-and-a-half-day workshop will cover cryo-ET workflows from sample preparation on EM grids to data processing and analysis. The workshop will be a mixture of lectures and practical sessions. Participants will get hands-on experience in sample preparation on EM grids (micropatterning)\, sample freezing (plunge freezing and high-pressure freezing)\, cryo-correlative light and electron microscopy (cryo-CLEM)\, cryo-focused ion beam milling (cryo-FIB)\, cryo-TEM tiltseries data collection\, and data processing and analysis (MotionCor2\, IMOD\, Dynamo). \n\n\n\nIt is compulsory that applicants have previous cryo-EM experience either with single-particle or already with cryo-tomography. \n\n\n\nThis workshop is supported by SciLifeLab\, CryoNET and Umeå University and organized by the Umeå Centre for Electron Microscopy (UCEM). \n\n\n\nContact email: erin.schexnaydre@umu.se
URL:https://www.scilifelab.se/event/workshop-for-cryo-electron-tomography/
LOCATION:KBC Building Umeå\, Linneaus Väg 6\, Umeå
CATEGORIES:Course
ATTACH;FMTTYPE=image/jpeg:https://www.scilifelab.se/wp-content/uploads/2020/11/CryoEM.jpg
ORGANIZER;CN="UCEM Ume%C3%A5":MAILTO:hussein.haggag@umu.se
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