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Single particle Cryo-EM image processing

National course open for PhD students, postdocs, researchers and other employees in need of cryo-EM data analyses skills within all Swedish universities.

The course is organized by the SciLifeLab Cryo-EM core facility.


This course provides a theoretical and practical introduction to cryo-EM image processing. It consists of morning lectures and computational image processing practicals in the afternoon. It focuses mainly on single particle cryo-EM data analysis and will not cover cell tomography.  The course is aimed to PhD student or researchers in general that already have cryo-EM data or are planning a cryo-EM single particle study in the near future. Upon completion of the course, attentive participants will be able to plan cryo-EM experiments and run most common cryo-EM image analyses from single particles to 3D classification and refinement.

Apply here

Important dates 

Application open: May 30

Application deadline: September 10

Acceptance confirmation: September 12

If you do not receive information according to the above dates please contact:

Course organizer: Marta Carroni.

Location: Auditorium Air and Fire, and room Milky Way

Course content

  • Concepts of TEM and image formation
  • EM-sample preparation
  • Concepts of Fourier analysis and CTF
  • Data acquisition: what to consider
  • Cryo-electron microscopy with the Volta phase
  • Particle selection
  • 3D volume refinement
  • Model building
  • Subtomogram averaging

Course fee

The course contains a voluntary social program for networking purposes (includes the coffee, lunches and one course dinner with teaching experts). A course fee of 1800 SEK will be invoiced to accepted participants that attend the social program, after completion of the course. NOTE – we cannot invoice individuals.

Entry requirements

Participants must be PhD students, postdocs or PI that are or are going to be directly involved in cryo-EM data processing for structure determination. Previous knowledge of cryo-EM and/or having  an ongoing cryo-EM project is desirable.

Selection criteria

Among the applicants, 30 participants will be selected based on correct entry requirements, motivation to attend the course, as well as gender and geographical balance.