Advanced Molecular Technology and Instrumentation for Proteome Analyses
National course open for SciLifeLab facility users and potential facility users, such as PhD students (prioritized), postdocs, researchers and other employees in need of proteomics skills within all Swedish universities.
To attend, the course participants must have background in the fields of biology, medicine, biochemistry or similar.
Response to accepted participants: June 21
Responsible teachers: Maria Hammond, Masood Kamali-Moghaddam, Cecilia Lindskog-Bergström, Sara Lind, Ganna Shevchenko
If you don’t receive information according to the dates above, contact email@example.com
For questions about the course curriculum, contact: firstname.lastname@example.org
The course offers a social program with all lunches and some coffee breaks included. Attendance is recommended, as it provides excellent opportunities for networking, but it is not mandatory.
Social program participants will be sent an invoice for the cost of the food, SEK 1800, after the course has been completed. NOTE – Uppsala University cannot invoice individuals.
The aim of the course is to introduce scientists to novel and advanced technologies for proteome analysis offering powerful tools in basic research and diagnostics. Both affinity- and non-affinity-based methods for protein detection will be covered. Mass spectrometry will be used for unbiased and high-throughput protein discovery and characterization; different variants of proximity ligation assays (PLAs) will be used for sensitive detection of proteins and their interactions and modifications in solution or in situ; and the participants will be introduced to histopathology methods including tissue microarray production, immunohistochemistry and slide scanning, using techniques, reagents and sample resources from the Human Proteome Atlas project.
The course will provide insight into state-of-the-art technologies for proteome analyses in research and medicine via advanced molecular technologies, reagent resources and instrumentation. The course will convey, by lectures and hands-on training, information about established and emerging methods and technologies relevant in basic research and diagnostics. Affinity- and non-affinity-based methods for protein analyses will be covered.
Topics covered will include:
- In situ PLA
- Solid-phase PLA
- Mass spectrometry
- HPA database
- Pathways analyses
The theoretical moments will include lectures, seminars and discussions, and they will be combined with practical moments including introduction to mass spectrometry, solid phase- and in situ PLA, and immunohistochemistry with tissue microarray production and image analysis.
The course participants must have background in the fields of biology, medicine, biochemistry or similar.