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CBCS course: Assay development in High Throughput Screening (Umeå)

Venue: Laboratories for Chemical Biology Umeå (LCBU), Umeå University, Umeå

Date: 16-20 November 2015    Register before 2015-10-26!

Responsible department: Department of Chemistry, Umeå University

Level: Advanced.

Content: The course gives a practical basis for assay development in high throughput screening. High throughput screening (HTS) is a technique used to identify biologically active small organic molecules through screening of compound libraries with the help of robust test systems and advanced instrumentation. The course includes assay development on a given enzyme and its substrate, however, all aspects of assay development applicable to both target-based assays and to cell-based assays will be covered. The course covers which parameters that are of importance for optimization of an assay. These different parameters will be tested empirically in the laboratory and their significance calculated using Z´. During the course a small-scale screen will be executed. The students will thereby be given knowledge about the importance of good screening logistics and gain insight into the amount of data generated and handling thereof. Hits from the screen will be selected and the process of validation will be covered. During the course the students will be introduced to instrumentation commonly used in high throughput screening such as liquid handling instrumentation and plate readers.


  • Bachelor degree (120 ECTS) in chemistry, molecular biology, pharmacology or equivalent. English knowledge corresponding to the Swedish upper secondary education English B.
  • The theoretical course: Introduction to high throughput screening (2 ECTS), which can be taken at your convenience. See the course web page for more information.

Registration: Before 2015-10-26. Send an e-mail, including a letter with a motivation for taking the course, to Stina Lindberg to register. The number of participants is limited.



Image courtesy of the LCBU website