Chemical Proteomics 2018
National course. The course is open for current and potential facility users such as PhD students, postdocs, group leaders and core facility staff in need of proteome-wide proteomics skills with aims in drug discovery and proteomics, within all Swedish universities.
This course is supported by SciLifeLab and run by the SciLifeLab Chemical Proteomics & Proteogenomics facility.
Still a few places left, registration is possible until October 15 on a first come, first served basis
Application open: July 30
Prolonged application deadline: October 15
Confirmation to accepted students: October 10
Responsible teachers: Massimiliano Gaetani, Roman Zubarev
If you don’t receive information according to the time period above, contact firstname.lastname@example.org.
A course fee of 1300 SEK will be invoiced to accepted participants (includes the coffee, lunches and course dinner). NOTE – SciLifeLab cannot invoice individuals.
This course will cover most updated chemical proteomics strategies available for identification and characterization of proteome-wide compound-target interactions and mechanisms of action. The course will show the major approaches that our facility uses to provide identification of drug target(s) and mechanism of action and to characterize them. The course will also offer research lectures of international level.
Topics will include:
- Introduction on mass spectrometry-based and quantitative proteomics approaches
- Data analysis and statistical analysis of quantitative proteomics data
- Traditional methods of chemical proteomics using molecular baits
- Modern and unbiased proteomics methods using drug/compound target molecules, by using protein extracts and cultured cells: a) thermal protein profiling (TPP), based on ligand inducted changes of protein thermal stability; b) Functional identification of target by expression proteomics (FITExP), based onto most relevant and specific responses of cell proteome to drug treatment for the identification of targets and potential keys of the drug´s efficacy.
- Data functional analysis and data interpretation.
- Elucidation of the interaction interface and mapping of the binding site of a drug with its target protein by Hydrogen/Deuterium (H/D) exchange mass spectrometry (HDX MS).
- Knowledge of basic in vitro assays using cells for drug/compound testing
- Basic knowledge in experimental design and statistical methods
- Basic knowledge in proteomics analysis
- Practical interest into unbiased identification and analysis of the interaction between a drug/compound of interest and its protein target(s), and/or into unbiased discovery of the key proteins and their related mechanism of cell responses upon drug/compound treatment.
Due to space constraints, there is a maximum number of allowed participants. If we receive more applications, participants will be selected based on several criteria. Selection criteria include correct entry requirements, motivation to attend the course as well as gender and geographical balance.