Cryo-EM sample preparation and data collection


Important dates

  • Application open: (2 months ahead of start)
  • Application deadline: (3 weeks ahead of start)

Responsible teachers: Linda Sandblad and Thomas Heidler

Contact information for questions regarding the course:

thomas.heidler@umu.se or linda.sandblad@umu.se

Course fee

No fee, Coffee, cookies and fruit will be served (no lunch included).

Course content

During a 3-day course you will learn the basic theory and get introduced to the practical skills required to work with cryo-EM methods. The basic functions and technical properties of a transmission electron microscope used for cryo-EM will be introduced. You will discuss challenges and practice cryo-EM sample preparation. Different methods for data collection will be introduced; demonstrations of single particle and tomography methods and software at Titan Krios, the high-end instrument for cryo-EM at SciLifeLab in both Stockholm and Umeå. Approaches for initial image processing will be introduced as a computer tutorial. The course will host 15 participants, PhD students and Postdocs have priority. Prerequisite is a basic knowledge in EM and/or structure biology. Organized by the SciLifeLab cryo-EM facility at Umeå Core facility for Electron Microscopy. Please, only apply if you have time to participate for 3 full days.

Course literature

A final schedule will be send out to the participants in advance. Other course material will be provided at the beginning of the course.

Entry requirements

Prerequisite is a basic knowledge in EM and/or structure biology. PhD students and Postdocs with an ongoing or future project are prioritized. It is therefore important to briefly describe relevant projects when applying to this course.