Hands on immunofluorescence


National course. The course is addressed to PhD students, postdocs, researchers and other employees within life science at any Swedish university, that want to learn more about fluorescence microscopy and antibody based imaging. This is a practical course so applicants should have some background in lab work. Applicants with a desire to apply imaging in their projects will be prioritized.

This course is supported by SciLifeLab and run by the SciLifeLab Cell Profiling facility and by the Human Protein Atlas (HPA) Cell Atlas team, in Stockholm.

Apply here

Important dates

  • Application open: May 10
  • Application deadline: July 6
  • Confirmation to the applicants: July 16
  • Location: Milky Way

If you don’t receive information according to the dates above, contact education@scilifelab.uu.se and david.gotthold@scilifelab.se

Responsible teacher:

Charlotte Stadler, Head of Cell Profiling Facility
Contact information for questions regarding the course:charlotte.stadler@scilifelab.se

Course fee

The course contains a voluntary social program for networking purposes (includes the coffee, lunches and one course dinner with teaching experts). A course fee of 1800 SEK will be invoiced to accepted participants that attend the social program, after completion of the course. NOTE – we cannot invoice individuals.

Course content

This course is organized by the Cell Profiling Facility and the Human Protein Atlas (HPA) Cell Atlas team.
The purpose of the course is to get experience from preparing samples for immunofluorescence and learn about fluorescence microscopy and how to acquire images from confocal microscopes. The course will combine theory and hands on work by performing a typical immunofluorescence workflow.
Participants will start in the cell lab with cell culturing and seeding, perform immunostaining with three markers and a target protein of choice (if applicable), and image the samples using a confocal microscope. At the microscope the concept of fluorescence will be demonstrated and some exercises will be done to illustrate how confocal microscopy and fluorescence microscopy works. The course will also cover annotation of immunofluorescence images and students will get practice on annotating images acquired.
All practical work will be done in smaller groups of 4 students in each.

Course literature

A few articles will be provided to accepted participants after registration dead–line and protocols for the practical work will be distributed.

Entry requirements

The course is addressed to PhD students, postdocs, researchers and other employees within life science at any Swedish university, curious about immunofluorescence and fluorescence microscopy.
Because of hands-on work the course is limited to 16 participants. Applicants should have some experience in cell culturing and motivate why they would benefit from the course. Applicants with an ongoing project or a defined future project that would benefit from fluorescence imaging will be prioritized, especially those lacking access to fluorescence microscopes in their lab.