Super-Resolution fluorescence microscopy (3 Hp, SK3514)


The course aims to provide all participants with training in the fundamental theoretical and experimental aspects of super-resolution fluorescence microscopy. 

Scientific tutorials will introduce fundamental and advance aspects of super-resolution fluorescence microscopy. Experimental hands-on session will further deepen the understanding and applicability of super-resolution microscopy. 

As the name implies, super-resolution fluorescence microscopy is a field in which novel optical approaches and single molecule sensitive fluorescence methods are applied to answer question in cellular biology via nanoscale biological imaging. 

Syllabus 

Lectures / seminars:

Introduction to available super-resolution fluorescence microscopy techniques (STED/PALM/STORM/SIM) and their applicability. Practical hands-on sessions on commercial super-resolution microscopy platforms, including advance imaging analysis.

Prerequisites 

Registered as PhD-student and submission of ALM access proposal (see link below) www.scilifelab.se/facilities/alm/

Requirements 

Literature study (1 university credit) Laboratory work (1 university credit) Examination (1 university credit)

Required reading 

Scientific articles (distributed)

Contact: Docent Hans Blom, Email: hblom@kth.se, 08-52481214

Department of Applied Physics, Royal Institute of Technology, Stockholm

The lectures of the course will be carried out at Karolinska Institute campus in Solna

(Lecture hall Inghesalen; floor 2, Tomtebodav 18A). Session with meet the experts and demos will be at the Advance Light Microscopy facility at Science for life laboratory, Tomtebodav 23A in Solna.

Last application date: 2014-05-05

Goals 

After the course all participants should have acquired the following knowledge/skills: 

  • Fundamentals of super-resolution microscopy
  • Practical implementation of each super-resolution technique
  • Analysis approaches in advanced imaging
  • Necessary conditions regarding sample preparations
  • Initial acquaintance of using commercial super-resolution microscopes
  • Present and critically discuss own scientific work and how to apply super-resolution fluorescence microscopy to individual projects

Selection
In the case of an over-booked course (max 20) selection will be based on the proposals.

Exam (grades) 
The super-resolution fluorescence microscopy course ends with a take-home exam (project proposal writing) where each PhD-student addresses how to apply the techniques to their own research.

The grade of the course is either pass or fail.

Description in PDF: Super-Resolution fluorescence microscopy