Biochemical and Cellular Screening

National facility

The Biochemical and Cellular Screening facility offer high throughput microplate based biological screening methods, both biochemical and cellular. The facility offers screening of activity for newly synthesized molecules in order to drive structure-activity explorations thereby enabling the next round of design of new molecules. The facility also provides high throughput RNAi knock-out technology for drug discovery projects.

SERVICES

  • High throughput.
    • Cell based assays and screens
    • Biochemical assays and screens
    • Phenotypical cell assays and screens
    • RNAi knock out genomic and customized  screens
  • Target validation.
  • Cellular profiling of drugs.
  • In vitro drug toxicity expertise.

EQUIPMENT

  • Labcyte 550 non-contact liquid handler.
  • PerkinElmer Janus 3 arm liquid handler.
  • Envision plate reader.
  • Biotec cell washer, flexdrop and wellmate dispensers.
  • Fully equipped cell room for cell cultivation.
  • Chemical and 2 human genomic siRNA libraries.

RECENT PROJECTS

1: Gad H, Koolmeister T, Jemth AS, Eshtad S, Jacques SA, Ström CE, Svensson LM, Schultz N, Lundbäck T, Einarsdottir BO, Saleh A, Göktürk C, Baranczewski P, Svensson R, Berntsson RP, Gustafsson R, Strömberg K, Sanjiv K, Jacques-Cordonnier MC, Desroses M, Gustavsson AL, Olofsson R, Johansson F, Homan EJ, Loseva O, Bräutigam L, Johansson L, Höglund A, Hagenkort A, Pham T, Altun M, Gaugaz FZ, Vikingsson S, Evers B, Henriksson M, Vallin KS, Wallner OA, Hammarström LG, Wiita E, Almlöf I, Kalderén C, Axelsson H, Djureinovic T, Puigvert JC, Häggblad M, Jeppsson F, Martens U, Lundin C, Lundgren B, Granelli I, Jensen AJ, Artursson P,  Nilsson JA, Stenmark P, Scobie M, Berglund UW, Helleday T. MTH1 inhibition eradicates cancer by preventing sanitation of the dNTP pool. Nature. 2014 Apr 10;508(7495):215-21.

2: Schmidt L, Kling T, Monsefi N, Olsson M, Hansson C, Baskaran S, Lundgren B, Martens U, Häggblad M, Westermark B, Forsberg Nilsson K, Uhrbom L, Karlsson-Lindahl L, Gerlee P, Nelander S. Comparative drug pair screening across multiple glioblastoma cell lines reveals novel drug-drug interactions. Neuro Oncol. 2013 Nov;15(11):1469-78.

3: Johansson HJ, Sanchez BC, Mundt F, Forshed J, Kovacs A, Panizza E, Hultin-Rosenberg L, Lundgren B, Martens U, Máthé G, Yakhini Z, Helou K, Krawiec K, Kanter L, Hjerpe A, Stål O, Linderholm BK, Lehtiö J. Retinoic acid receptor alpha is associated with tamoxifen resistance in breast cancer. Nat Commun. 2013;4:2175.