The High Throughput Genome Engineering (HTGE) facility provides affordable access to high throughput functional genomic screens using the CRISPR/Cas9 system in cell lines. Pooled CRISPR/Cas9 screening enables parallel interrogation of thousands to tens of thousands of genes for involvement in biological processes of interest. HTGE provides access to verified lentiviral CRISPR guide libraries for whole genome and more targeted loss- and gain of function studies (CRISPR knock-out, CRISPR inhibition, CRISPR activation). We also offer generation of stable Cas9-expressing lines in users’ cells of interest. CRISPR gene perturbation followed by single cell RNASeq is under development together with Eukaryotic Single Cell Genomics (ESCG), and we are developing base-editing approaches to mutagenize sequences to, for example, characterize protein-protein or protein-drug interactions. HTGE foremost aim is to offer state-of-the-art services, and we are happy to implement new screening technologies in collaboration with interested clients. HTGE supports the Swedish research community with CRISPR screening projects from planning to data analysis.
Our local branch, Karolinska Genome Engineering (KGE), performs precision edits in cell lines, such as knock-out, knock-in, deletions, point mutations, etc. KGE collaborate with the Karolinska Center for Transgene Technologies (KCTT) to offer CRISPR technology in mouse, and is implementing a pipeline with the iPSC core facility for precision editing of iPSCs.
From left to right: Soniya, Jenna, Bernhard, Georgia, Allegra. Photo: Christos Coucoravas
Your project is out of the box? All the better, we would love to hear from you!
The precision and accuracy of CRISPR/Cas9 screens is dramatically improved by the incorporation of inert, random sequence labels (RSLs) into CRISPR guides.
HTGE, Biomedicum 9B
171 77 Stockholm
171 65 Solna
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