Genome Engineering Zebrafish

The Zebrafish facility provides an infrastructure and individually tailored support for projects utilizing this popular model system for vertebrate development and disease.
Zebrafish embryos are transparent and develop outside the mother’s body, which greatly facilitates manipulation and imaging of biological processes. The facility allows researchers to take advantage of the unique features of the zebrafish model system, and provides initial advice on feasibility and experimental design as well as running support over the course of the project.

Read more about the facility at Uppsala university website.

Services

  • CRISPR/Cas9 induced loss of function zebrafish line generation in ourselection of readout models
  • Development and analysis of CRISPR/Cas9 induced disease models
  • Zebrafish image informatics (Zii); GEZ and the BioImage Informatics Facility capability allowing for automated, high through-put fluorescent imaging service based on the Vertebrate Automated Screening Technology, Union Biometrica combined with large scale analysis of imaging data
  • Genotyping by fluorescent PCR (fin-clip and embryos)
  • Techniques for reverse and forward genetic experiments
  • Possibility to involve specialists at all levels in individual projects
  • Project planning/management
  • Visiting hours, workshops and education
  • Access to a well-equipped facility
  • Embryo and adult fish production
  • High quality animal husbandry and strain maintenance

Applications

  • Development of disease models by introducing mutations in zebrafish genes
  • homologous to human disease genes
  • Target validation of candidate genes and/or small compounds
  • Investigation of gene function in the developing zebrafish
  • Imaging of organism development and tissue morphology and function

Equipment

  • Fish facility, including tank systems, fish lines, workstations, incubators and injectors
  • Large scale robotic expression analysis Intavis InsituPro Vsi
  • Microscopy: Variety of microscopy equipment including bright field, fluorescent microscopes and VAST (Vertebrate Automated Screening Technology) BioImager Platform with Large Particle Sampler allowing for sampling and re-dispensing of zebrafish larvae (https://www.unionbio.com/vast/)

Mailing Address

Uppsala University
Evolutionsbiologiskt Centrum (EBC)
Norbyvägen 18 A
752 36 Uppsala

Visiting Address/Deliveries

Uppsala University
Evolutionsbiologiskt Centrum (EBC)
Norbyvägen 18 A
752 36 Uppsala

This website uses cookies to improve your experience. We'll assume you're ok with this, but you can opt-out if you wish. Ok!Read More
Privacy & Cookies Policy

Privacy Overview

This website uses cookies to improve your experience while you navigate through the website. Out of these cookies, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. But opting out of some of these cookies may have an effect on your browsing experience.
Necessary
Always Enabled

Necessary cookies are absolutely essential for the website to function properly. This category only includes cookies that ensures basic functionalities and security features of the website. These cookies do not store any personal information.

Non-necessary

Any cookies that may not be particularly necessary for the website to function and is used specifically to collect user personal data via analytics, ads, other embedded contents are termed as non-necessary cookies. It is mandatory to procure user consent prior to running these cookies on your website.