PLA and Single Cell Proteomics
National facility
The facility employs the proximity ligation assays (PLA) for high-performance DNA-assisted protein analyses. DNA oligonucleotides conjugated to antibodies are used as affinity reagents, and upon pairwise or trio binding of such reagents to a target protein a reporter DNA strand is formed via ligation for detection in solution or in situ. The facility offers services where PLA is applied in cells or in tissue sections to image with enhanced specificity the presence of specific proteins, or to analyze interactions and posttranslational modifications of investigated proteins to e.g. characterize acts of protein signaling in tumor tissues, or to evaluate effects of drug treatment, etc. The facility further offers services to establish highly sensitive assays for proteins in solution phase.
SERVICES
- Validation, optimization and assay development using client-selected antibodies
- In situ PLA. In situ PLA is applied for detecting proteins and their interactions and post-translational modifications using either client-selected antibodies or previously validated antibodies from a continuously updated list
APPLICATIONS
- Visualization and quantification of proteins, their interactions and post-translational modifications in cells and tissues
- Analyses of biological pathways and cell signaling
- Quantitative analyses of the effect of drug candidates on protein expression, interactions and modifications
EQUIPMENT
- Robotic pipetting dispenser
- Fluorescence scanner
- Confocal microscope
- Epi-fluorescence microscopes
