Swedish NMR Centre (SNC)

National facility

snc_nmrhallen_700w_300h

The Swedish NMR Centre (SNC) is an international, national and local research infrastructure that provides access to state-of-the-art NMR instrumentation and methodology, specifically within

  • Structural biology
  • Metabolomics
  • Chemical biology and small molecule NMR
  • Diffusion, microimaging and MAS NMR

SNC comprises two nodes, in Gothenburg and Umeå, with complementary competences. The Gothenburg node has much focus on structural biology and metabolomics studies on liquids, whereas Umeå can cater for solid-state NMR metabolomics studies. Both nodes have capability for fragment-based screening. For more information, please visit us at SNC Gothenburg or SNC Umeå.

EQUIPMENT

NMR spectrometers and main probe configuration


Gothenburg

  • Oxford 900 pumped magnet, Bruker Avance III HD spectrometer, 5 mm TCI cryoprobe
  • Oxford 800 pumped magnet, Bruker Avance III HD spectrometer, cooled SampleJet sample changer, 3 mm TCI cryoprobe
  • Oxford 800 magnet,  Bruker Avance III HD spectrometer, SampleJet sample changer, 5 mm TXO cryoprobe
  • Varian INOVA 600 spectrometer, 3.2 mm MAS solids probe
  • Varian INOVA 600 spectrometer, 5 mm cold probe
  • Bruker 600 Avance III HD spectrometer, 5 mm diffusion probe
  • Varian Inova 500 spectrometer, 5 mm RT probe

Umeå

  • Bruker 850 Avance III HD spectrometer, 5mm TXI cryoprobe, sample changer
  • Bruker 600 MHz Avance III, cooled SampleJet sample changer
  • Bruker 500 MHz Avance III

 

Sample preparation equipment


Gothenburg

  • Bruker SamplePro L liquid handling robot
  • Agilent Bravo liquid handling robot

Umeå

  • Bruker SamplePro L liquid handling robot

 

RECENT PROJECTS

Structural biology

  • Unnerståle, S., M. Nowakowski, V. Baraznenok, G. Stenberg, J. Lindberg, M. Mayzel, V. Orekhov, and T. Agback. Backbone Assignment of the MALT1 Paracaspase by Solution NMR. PLoS ONE, 2016. 11(1): p. e0146496-9.
  • Edwin, A., C. Persson, M. Mayzel, S.N. Wai, A. Öhman, B.G. Karlsson, and A.E. Sauer‐Eriksson. Structure of the N‐terminal domain of the metalloprotease PrtV from Vibrio cholerae. Protein Science, 2015. 24(12): p. 2076-2080.
  • Rosenlöw, J., L. Isaksson, M. Mayzel, J. Lengqvist, and V.Y. Orekhov, Tyrosine Phosphorylation within the Intrinsically Disordered Cytosolic Domains of the B-Cell Receptor: An NMR-Based Structural Analysis. PLoS ONE, 2014. 9(4): p. e96199.

Metabolomics

  • Stenson, M., Pedersen, A., Hasselblom, S., Nilsson-Ehle, H., Karlsson, B.G., Pinto, R. and Andersson, P-O. Serum nuclear magnetic resonance-based metabolomics and outcome in diffuse large B-cell lymphoma patients – a pilot study. Leukemia & Lymphoma, 2016, http://dx.doi.org/10.3109/10428194.2016.1140164
  • Andersson Hall, U., Edin, F., Pedersen, A. and Madsen, K. Whole-body fat oxidation increases more by prior exercise than overnight fasting in elite endurance athletes. Applied Physiology, Nutrition, and Metabolism, Published on the web 17 December 2015, 10.1139/apnm-2015-0452

Data sampling, signal processing and data analysis

  • Qu, X., M. Mayzel, J.F. Cai, Z. Chen, and V. Orekhov. Accelerated NMR Spectroscopy with Low‐Rank Reconstruction. Angewandte Chemie International Edition, 2015. 54(3): p. 852-854.