Last updated: 2022-03-01
Content Responsible: Scilifelab Administration ()
Human Antibody Therapeutics
The Human Antibody Therapeutics unit supports the development of therapeutic antibodies. The unit will assist by isolating human antibodies from in-house constructed IP free phage libraries towards target proteins as suggested by the partner PI. It will be of critical importance that the partner PI has substantial knowledge and know-how of the target protein/biological system, either from a clinical or basic scientific perspective. The objective is to deliver a reasonable number of candidate clones for further in vivo characterization in collaboration with the partner PI.
Services
Generation of well-characterized human antibodies for therapy
Selection and cloning of monoclonal, high-affinity human antibody fragments from IP free phage display libraries
Re-cloning, expression and purification of isolated antibodies as full-length format IgG molecules
Initial characterization of the isolated antibodies, including e.g. DNA/amino acid sequence, binding specificity and selectivity, affinity, stability, aggregation, epitope mapping
Assessment of function and biological potency of the antibodies using in vitro assays
Collaborate in the design of relevant assays for biological potency of the antibodies, and in the design of proof-of-concept studies
Consulting at the pre-project proposal stage with potential partner PIs on project design
Equipment
Highly diverse IP free human antibody phage libraries
Fully equipped pro- and eukaryotic cell culture laboratories
Protein purification ÄKTA systems
Automated liquid handling VANTAGE Hamilton robot
SPR Biacore 8K
ELISA work station
KingFisher Flex system
iQue Plus flow cytometer
NanoDSF Prometheus
Isothermal titration calorimeter
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