Protein Expression and Characterization

Infrastructure Unit

Contact

Anders Olsson

Recent user publications

The publications in this database are the result of research conducted at the units of SciLifeLab – both in user projects and technology development.

In Vitro and In Vivo Development of a β-Lactam-Metallo-β-Lactamase Inhibitor: Targeting Carbapenem-Resistant Enterobacterales.

BK. Peters, N. Reddy, M. Shungube, (…), HG. Kruger, T. Naicker, T. Govender,

ACS Infect Dis9 (3) 486-496

Monitoring drug-target interactions through target engagement-mediated amplification on arrays and in situ.

RA. Al-Amin, L. Johansson, E. Abdurakhmanov, (…), P. Artursson, T. Lundbäck, U. Landegren,

Nucleic Acids Res.50 (22) e129

Autoantigenic properties of the aminoacyl tRNA synthetase family in idiopathic inflammatory myopathies.

C. Preger, A. Notarnicola, C. Hellström, (…), H. Persson, S. Gräslund, PJ. Jakobsson,

J. Autoimmun.134 102951

Organic Base-Mediated Carboxylation of (Hetero)aromatic Compounds Using Supercritical Carbon Dioxide (scCO2)

T. Govender, LC. Chetty, HG. Kruger, PI. Arvidsson, T. Naicker,

Synthesis54 (21) 4827-4833

Personnel

Per-Åke Nygren, Platform Scientific Director
Anders Olsson, Head of Unit
Yasmin Andersson, Senior Scientist
Leif Dahllund, Senior Scientist
Esmeralda Woestenenk, Senior Scientist

SciLifeLab / Services / Our infrastructure / Protein Expression and Characterization

Protein Expression and Characterization

The Protein Expression and Characterization unit provides recombinant proteins needed for biochemical screening and therapeutic applications as well as antigens for human antibody production using bacterial and mammalian expression systems. The unit also houses competence and machinery to characterize purified proteins in detail, like binding, stability, and aggregation tendency.

Services

Delivery of pharmaceutical target proteins for screening and MOA studies including:
- Construct design, cloning and expression analysis
- Expression in E. coli, insect cells, transient and stable pool mammalian cells up to 20L scale
- Purification and biophysical characterisation of proteins
Expression, purification and characterisation of biopharmaceutical candidates for Proof of Concept and MOA studies
qPCR expression analysis
Mammalian cells for screening
Thermal shift screens (DSF)
Support in biochemical screening assay design and setup

Equipment

Watch our cells grow!

Fully equipped lab for protein expression and purification
ambr15 Microbioreactor
Light Cycler 480 II
Isothermal calorimeter
Biacore T200
Liquid handling robot
Prometheus NT.48 with Backreflection Optics
Malvern DLS