Postdoctoral research fellow in Genome Architecture and Fragility in Aging

Published: 2018-05-14


A postdoctoral position is now available in the Bienko and Crosetto Laboratories (https://bienkocrosettolabs.org/) to investigate the link between genome architecture and fragility in the context of human aging.

Research environment
The Bienko and Crosetto labs are two highly integrated research groups working on genome architecture (Bienko group) and genome fragility (Crosetto group) in healthy and disease conditions. We pioneered BLESS, the first method for direct genome-wide localization of DNA double-strand-breaks DSBs (Crosetto et al., Nature Methods 2013), and recently further advanced the technology by developing BLISS (Yan et al., Nat Commun 2017; Mirzazadeh et al., Meth Mol Biol 2018). We have also developed GPSeq, a new method based on massively parallel sequencing that enables to measure the radial nuclear position of thousands of genomic loci simultaneously (manuscript in preparation). We are applying BLISS and GPSeq, together with a variety of other genome-wide techniques (RNA-seq, HiC), to investigate the relationship between genome architecture and fragility in various contexts.

Our labs are located at the Science for Life Laboratory (SciLifeLab) situated at the Karolinska Institute campus in Stockholm. SciLifeLab is an interdisciplinary center for molecular biosciences with focus on health and environmental research, bringing under the same roof groups from four universities: Karolinska Institutet, KTH Royal Institute of Technology, Stockholm University and Uppsala University. The center features state-of-the-art technology platforms, including next-generation sequencing, high-throughput histology, super-resolution microscopy, proteomics, image analysis, and bioinformatics.

The successful candidate will join an interdisciplinary and dynamic team of international researchers, including clinicians, biologists, biotechnologists, engineers, computer scientists, and physicists. Our mission is to transform the way we understand complex biological phenomena and diseases such as cancer, by integrating next-generation sequencing technologies, single-molecule microscopy methods, and advanced computational tools.

Duties
The project is aimed at studying DSBs, DSB-associated mutations, and genome architecture in primary human cells obtained from individuals of different age, and from the same individual at different ages, using a combination of ‘omic’ approaches.

Specific tasks include:

  1. Performing BLISS, GPSeq, HiC, and RNA-seq in primary fibroblasts obtained from the Coriell Repository (https://www.coriell.org/)
  2. Establishing promoter-capture probes for targeted deep sequencing of fragile promoters
  3. Working alongside a senior bioinformatician to analyze and interpret the data

Entry requirements
A person is eligible for a position as postdoctoral research fellow if he or she has obtained a PhD no more than seven years before the last date of employment as postdoc.

A clearly demonstrated prior experience on genome-wide methods such as ChIP-seq, RNA-seq, HiC or similar methods, and their related analytical approaches is mandatory. A strong affinity for molecular methods development and prior experience with new protocols development, optimization, and troubleshooting is necessary. A strong motivation to work in a highly interdisciplinary and collaborative environment, and willingness to interact with scientists with computational background is required. Experimentalists with a strong motivation to work alongside theoreticians and to learn analytical and programming skills are strongly encouraged to apply.

Application process
An employment application must contain the following documents in English:

  • A complete curriculum vitae, including date of the thesis defence, title of the thesis, previous academic positions, academic title, current position, academic distinctions, and committee work
  • A complete list of publications
  • A summary of current work (no more than one page)
  • Verifications for crediting of illness, military service, work for labour unions or student organisations, parental leave or similar circumstances
  • Verification from the thesis defence committee or the equivalent (only if the thesis defence is scheduled within three months after the application deadline)

The application is to be submitted on the MyNetwork recruitment system.