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Advanced Light Microscopy

National facility

ALM for web

The mission of the Advanced Light Microscopy (ALM) facility is to give open-access to state-of-the art superresolution fluorescence microscopy for nanoscale biological visualization. The facility offers access to commercially available super-resolution techniques (STED, PALM/STORM, SIM). Collaborative project management and transfer of unique knowledge to individual researchers are supported nationally, including organization of workshops and courses in superresolution microscopy. Access also includes specific superresolution specimen preparation and data analysis support.

SERVICES

  • Super-resolution microscopy. Cellular, Thin tissue - fixed or living samples
    • please note that optimum resolutions are always sample dependent (cell culture density, selected probes, density of labels, environment, etc) and need individual testing for all supported projects
  • Specimen preparation. Sample optimization, fluorescent probes selection
  • Data analysis. Postprocessing of recorded data, deconvolution, visualisation
  • Quantitative analysis. Superresolved amount, topology and co-occurrence

EQUIPMENT

  • PALM/STORM. Zeiss Elyra 3D PALM.
  • STED. Leica SP8 3X STED and Leica SP5 NIR-STED
  • SIM. Zeiss Elyra 3D SIM

APPLICATIONS

  • Investigation of molecular architecture of sub-cellular entities.
  • Organization of proteins in cell biology with sub-diffraction precision.
  • Imaging of fluorescent protein localizations and dynamics in cultures of living cells.

RECENT PROJECTS

  • Visualization of synaptonemal complexes (Prof. Christer Höög, Karolinska Insitutet)
    doi: 10.1002/embj.201387329
  • Visualization of divisome in bacteria (Dr. Dan Daley, Stockholm University)
    doi: 10.1111/mmi.12534
  • Visualization of cellular junction proteins (Dr. Elena Vikström, Linköping University)
    doi: 10.1371/journal.ppat.1002953
  • Visualization of synaptic proteins (Dr. Linda Westin, Karolinska Institutet)
    doi: 10.1186/1471-2202-15-45
  • Visualization of synaptic proteins (Prof. Klas Kullander, Uppsala University)
    doi: 10.1016/j.biopsych.2014.07.017
  • Bacteria in the lymp node (Dr. Antonio Gigliotti Rothfuchs, Karolinska Insitutet)
    doi: 10.1371/journal.ppat.1005206
  • Cell generation in the human heart (Dr. Olaf Bergman, Karolinska Insitutet)
    doi: 10.1016/j.cell.2015.05.026
  • Compacting rolling circle amplification (Dr. Ola Söderberg, Uppsala University)
    doi: 10.1038/srep12317
  • RhoD association in Golgi and ER (Prof. Pontus Aspenström, Karolinska Insitutet)
    doi: 10.1016/j.yexcr.2015.02.023
  • Quorum sensing in macrophages (Dr. Elena Vikström, Linköping University)
    doi: 10.3389/fmicb.2015.00915
  • Glial origin of mesenchymal stem cells  (Dr. Igor Adameyko, Karolinska Institutet)
    doi: 10.1038/nature13536
  • Nanoparticle-stabalized micorsobubles for drug delivery  (Dr. Yrr Mörch, NTNU Trondheim, Norway)
    doi: 10.1002/cmmi.1639