Lukas Orre
Research Interests
Our research group focus is divided between two main fields:
Development and application of Mass Spectrometry (MS)-based proteomics methods
In the proteomics method development field, our aim is improving the analytical depth and quantitative accuracy in shotgun proteomics. In addition, we develop methods for functional proteomics such as analysis of post-translational modification of proteins, subcellular localization of proteins and drug response profiling at the protein level. Further, we are involved in projects where methods for proteogenomics are developed and applied, as well as methods for statistical analysis of MS data.
Cancer proteomics research aiming to improve the treatment of lung cancer patients
In the cancer proteomics field, our current research is aiming for improvement of cancer therapy, treatment prediction and combination therapy, specifically in the context of lung cancer. This research is based on in-depth analysis of the molecular response to targeted therapies in model cell lines for identification of candidate predictive biomarkers and effective combination therapies. For validation of candidate combination therapies, cell-based drug screening is used. For clinical evaluation of candidate biomarkers both in-depth profiling of clinical lung cancer samples and targeted assays are used.
Group Members
- Lukas Orre, PI
- Georgios Mermelekas, Senior Infrastructure Specialist
- Olena Berkovska, PostDoc
- Soumitra Marate, PostDoc
- Amare Wolide, PostDoc
- George Field, PhD student
- Irene Villanueva Sanz, PhD student
- Igor Schliemann, PhD student
Key Publications
Taner Arslan, Yanbo Pan, Georgios Mermelekas, Mattias Vesterlund, Lukas M. Orre and Janne Lehtiö.
SubCellBarCode: integrated workflow for robust spatial proteomics by mass spectrometry.
Nature Protocols. 2022 Aug;17(8):1832-1867. PMID: 35732783
Janne Lehtiö, Taner Arslan, Ioannis Siavelis, Yanbo Pan, Fabio Socciarelli, Olena Berkovska, Husen M. Umer, Georgios Mermelekas, Mohammad Pirmoradian, Mats Jönsson, Hans Brunnström, Odd Terje Brustugun, Krishna Pinganksha Purohit, Richard Cunningham, Hassan Foroughi Asl, Sofi Isaksson, Elsa Arbajian, Mattias Aine, Anna Karlsson, Marija Kotevska, Carsten Gram Hansen, Vilde Drageset Haakensen, Åslaug Helland, David Tamborero, Henrik J. Johansson, Rui M. Branca, Maria Planck, Johan Staaf, and Lukas M. Orre.
Proteogenomics of non-small cell lung cancer reveals molecular subtypes associated with specific therapeutic targets and immune evasion mechanisms.
Nature Cancer. 2021 Nov; 22 (2), 1224–1242. PMID: 34870237
Zhu Y, Orre LM, Zhou Tran Y, Mermelekas G, Johansson HJ, Malyutina A, Anders S, Lehtiö J.
DEqMS: A Method for Accurate Variance Estimation in Differential Protein Expression Analysis.
Mol Cell Proteomics. 2020 Jun;19(6):1047-1057. PMID: 32205417
Orre LM, Vesterlund M, Pan Y, Arslan T, Zhu Y, Fernandez Woodbridge A, Frings O, Fredlund E, Lehtiö J.
SubCellBarCode: Proteome-wide Mapping of Protein Localization and Relocalization.
Molecular Cell. 2019 Jan 3;73(1):166-182.e7. PMID: 30609389