Host cell systems biology and targets
This research area aims at creating capabilities for discovery and functional understanding of the virus interactome and host effects, and build capabilities for high-throughput (chemical and CRISPR) analysis of virus-infected cells. Furthermore, the projects aim to build cell models, assays and data.
This area consists of 11 individual projects, funded a total of 5,3 MSEK. Led by researchers at Uppsala University, KTH Royal Institute of Technology, Karolinska Institutet, Lund University and Umeå University.
Coordinator: Marjo Puumalainen, Karolinska Institutet
Scientific lead: Oscar Fernandez-Capetillo and Päivi Östling, Karolinska Institutet
Projects
Functional characterization of Covid19-host response using single-cell transcriptomics and CRISPR screens
Anna Överby Wernstedt, Umeå University
Immediate molecular response to SARS-CoV-2 infection
Jonas Klingström, Karolinska Institutet
Mapping SARS-CoV-2 host-pathogen interactions for drug repurposing
Ylva Ivarsson, Uppsala University
Viral-host interaction of SARS-CoV-2 protein corona
Maria Pernemalm, Karolinska Institutet
Profiling of host proteins associated to the envelope of SARS-CoV-2
Claudia Fredolini, KTH
Genetic screens to identify novel determinants of SARS-CoV-2 infection
Oscar Fernandez-Capetillo, Karolinska Institutet
Cellular thermal shift assay (CETSA) to identify host factors involved in SARS-CoV-2 entry and replication
Leo Hanke, Karolinska Institutet
Identification of host factors targeted by coronaviruses by Thermal proteome profiling
Oscar Fernandez-Capetillo, Karolinska Institutet
Creating synthetic biology tools to reveal the determinants of SARS-CoV-2 interactions with host cells
Erdinc Sezgin, Karolinska Institutet
Human precision cut lung slices as an ex vivo model for studying SARS-CoV2 infection and identifying potential therapies
Darcy Wagner, Lund University
Identifying the Achilles heel of SARS-CoV-2 by using gene scissors
Claudia Kutter, Karolinska Institutet