School of Engineering Sciences in Chemistry, Biotechnology and Health at KTH
KTH Royal Institute of Technology in Stockholm has grown to become one of Europe’s leading technical and engineering universities, as well as a key centre of intellectual talent and innovation. We are Sweden’s largest technical research and learning institution and home to students, researchers and faculty from around the world. Our research and education covers a wide area including natural sciences and all branches of engineering, as well as architecture, industrial management, urban planning, history and philosophy.
School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH) is one of the five schools of KTH and a strong constellation for research and education in the broader areas of health, environment, energy and materials. The School of Engineering Sciences in Chemistry, Biotechnology and Health conducts research and education in areas that cover several global challenges. Among them are the need for sustainable energy, health and aging population, climate change, sustainable production and working life, food production and clean water.
The Cell profiling group (PI Emma Lundberg) at SciLifeLab in Stockholm works on different projects revolving around spatial proteomics for cells and tissues. The group consists of three teams: research, HPA production and the Spatial Proteomics facility, but the teams are interlinked and share a common infrastructure. We are now looking for one research engineer for the facility and one research engineer for the production team.
The production team produces and maintains the subcellular section of the Human Protein Atlas (HPA) with the aim to systematically map the location of all human proteins in cells, tissues and organs. As a research engineer in this team, you will be localizing proteins at the subcellular level in cell lines by using immunofluorescence (ICC-IF) and confocal microscopy. The tasks mainly include laboratory work, but also some analytical tasks. In the lab, you will mainly do cell cultivation, immunostaining and microscopy. The analytical tasks include qualitative image analysis and curation of data for the Cell Atlas. You will work according to established SOPs (Standard Operating Procedures) and document your work in LIMS (Laboratory Information Management System).
The Spatial Proteomics National Facility is part of the newly established Spatial and Single Cell Biology Platform and part of the research infrastructure at SciLifeLab. The focus of the Spatial Proteomics unit is to offer highly multiplexed immunofluorescence of cells and tissue sections. Currently we work with the latest instruments from Akoya Biosciences (Phenocyler Fusion) Lunaphore (COMET and LabSat). As a national facility, much work will be to perform service projects for external users, but the position also includes internal research and development, for eg validating new antibody panels and development of new methods and protocols. Depending on the background and interest of the applicant, more time can be spent on image data analysis.
Production team: Previous experience in laboratory work, including common methods in molecular biology, cell cultivation and immunostaining of cells (ICC-IF). It is a great advantage if you have experience of confocal microscopy and image analysis. Previous work with Tecan liquid handling robots and/or documentation with the help of a Laboratory Information Management System (LIMS) is also advantageous.
Spatial Proteomics facility: Previous experience in multiplexed imaging methods, tissue sample preparation including sectioning, antibody conjugation and antibody validation for immunofluorescence is a strong advantage. Also knowledge in immunology, histology, pathology and/or quantitative image analysis and programming is an advantage.
Great emphasis will be placed on personal competency. As a person, you are reliable and a good team player that can also work independently and drive projects forward. You have excellent communication skills. You are organized, well structured and thorough.
For the position in the facility it is extra important that you show problem-solving abilities and are technical. For this position it is also important to be organized, quality focused and service minded.
You will find contact information to trade union representatives at KTH’s webbpage.
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Your complete application must be received by KTH no later than the last day of application, midnight CET/CEST (Central European Time/Central European Summer Time).