Cryo-EM sample preparation and data collection
October 20 @ 08:00 – October 22 @ 17:00 CEST
The purpose of the course is to prepare and train Cryo-EM unit users in sample preparation methods, introduce users to the image data acquisition workflow, expand knowledge about cryo-EM methods among researchers and show that everyone can learn how to use cryo-EM for structure and cell biology.
The course is open for unit users or potential unit users, such as PhD students, postdocs, and researchers within the life sciences who are curious and will profit from cryo-EM skills. Swedish and international course participants are welcome. To attend, the course participants must have experience in basic electron microscopy and be familiar with structure or cell biology.
This course is supported by SciLifeLab, CryoNET and Umeå University and organized by the SciLifeLab Cryo-EM unit, both UmU and SU nodes together.
Application should be submitted before September 17, 2021
Confirmation to accepted participants: September 22, 2021
(If you don’t receive information about your application before September 27, please contact firstname.lastname@example.org
Camilla Holmlund and Michael Hall
Teachers and course instructors
- Linda Sandblad
- Michael Hall
- Lars-Anders Carlson
- Camilla Holmlund
- Additional teachers t.b.a
A social program depending on the current corona restrictions will be communicated to the participants after acceptance.
A course fee of 800 SEK, including coffee and lunches, will be invoiced to accepted participants after acceptance.
This course will cover practical aspects of cryo-EM sample preparation and data acquisition.
- Basic theory of vitreous water, how to handle a cryo sample and consequences
- Plunge freezing methods, tools and cryo-transfer
- Sample and instrument requirements for single particle and tomography methods
- Image data acquisition, concept of low dose and the cryo-specific workflow
- Introduction to automatic data acquisition software, EPU, Tomography
- Introduction to cryo-EM image processing methods
The course consists of lectures, cryo lab work, demonstrations and practical exercises for all participants, hands-on experiences of microscopy operation and discussions. A detailed schedule will be provided to accepted participant.
Handouts and material online.
Priority will be given to researchers involved in project where the use of cryo-EM is needed, which are to be briefly described upon application.
The course will be held at an advanced level, assuming that participants have a master’s degree or equivalent in a technical life science discipline. It aims to be relevant for a broad research community, also to participants not working in research environments using cryo-EM today.
A maximum of 15 participants will be admitted. Selection criteria include correct entry requirements, motivation to attend the course as well as gender balance.
Participation in all parts of the course, lectures, lab, microscopy demonstrations and evaluation are mandatory. SciLifeLab and Umeå University will provide successful participants with a course certificate and a recommendation for 1 ECTS
Please note that SciLifeLab training courses do not provide any formal university credits.
The course content is estimated to correspond to a certain number of credits, however the estimated credits are just guidelines.If formal credits are crucial, the student needs to confer with the home department before course application, whether the course is valid for formal credits or not.