New Super-Resolution, Light-Sheet, and FCS-Methods at Scilifelab
October 14 @ 08:00 – October 25 @ 17:00 CEST
The Advanced Light Microscopy Facility, ALM, at Scilifelab gives a two-week PhD-level course on four new imaging and fluorescence spectroscopy techniques:
Depletion-based Super-Resolution Imaging
- STED-imaging, 2D and 3D, 30-50 nm resolution
- In Living Cells – MoNaLISA, 1-2 Hz, 100 x 100 mm field of view
MINFLUX
- Imaging with 3 nm resolution
- Tracking single molecules at 10 kHz
Light-Sheet Imaging
- Long time-lapse imaging of live model organisms with low phototoxicity
- Ultrafast volumetric imaging of cells with lattice light-sheet microscopy
FCS-Methods
- Measurements of concentrations, diffusion coefficients, molecular dynamics and interactions, in solution or living cells
- Cross-correlation, FRET-, STED-, and line-scan-FCS are covered
Lecturers:
Steven Edwards
Erdinc Sezgin
Ana Agostinho
Francesca Pennacchietti
Hans Blom
Stefan Wennmalm
Dates: Monday October 14th – Friday October 25th 2024
Credits: 3 hp for PhD students
Location: Gamma 3, Scilifelab, Solna
Registration: stewen@kth.se latest September 30th
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