RNA-seq data analysis


Uppsala University
Uppsala, Sweden

RNA-seq data analysis

March 11, 2024 @ 08:00 March 15, 2024 @ 17:00 CET

This workshop will introduce the best practice bioinformatics methods for processing and analyses of RNA-seq data via a series of  lectures and computer practicals.

Topics covered will include:

  • RNAseq quality control analysis
  • Mapping of short reads
  • Quantification of gene expression
  • Exploratory data analysis
  • Differential gene expression
  • Gene set enrichment analysis

More details about the course can be found at the previous year webpage: RNAseq course 2023

The course will follow similar structure and topics.

NOTE: in March 2024, the course will be a 5-day-course on-site in Uppsala.

Entry requirements

Required for being able to follow the course and complete the computer exercises:

  • Basic knowledge in Linux and R
  • Your own laptop with the following specs:
    • At least 6 GB of RAM.
    • Operating system Win10, OS X or Linux
    • At least 30 GB of free storage


  • Experience working with NGS data analysis or completed the NBIS workshop “Introduction to Bioinformatics using NGS data”
  • Experience working in R or completed the NBIS course “R Programming Foundations for Life Scientists”

Due to our best practice to have a high teacher to student ratio we have set the number of participants to a maximum of 20 participants. If we receive more applications, participants will be selected based on several criteria. Selection criteria include correct entry requirements, motivation to attend the course as well as gender and geographical balance.

Course fee

A course fee* of 3000 SEK for non-profit organisations will be invoiced to accepted participants. This includes lunches, coffee, snacks, and one course dinner.

*Please note that NBIS cannot invoice individuals

Important dates:

  • Application open: December 19, 2023
  • Application deadline: February 19, 2024

Responsible teacher/s:
Johan Reimegård, Julie Lorent, Nima Rafati

Contact information for questions regarding the course

Uppsala, Sweden

Last updated: 2024-02-13

Content Responsible: Hampus Persson(hampus.persson@scilifelab.uu.se)