Spatial analysis of gene expression in tissues
May 2 @ 08:00 – May 6 @ 17:00 CEST
Single-cell genomics technologies have become widely used to study heterogeneous and dynamic biological systems. In order to meet the growing demand for a deeper understanding of tissue profiling, a number of different spatial transcriptomics approaches have been developed and applied successfully.
The goal of this practical course is to provide participants with an overview of the different spatially resolved transcriptome mapping techniques, learn about the differences and advantages of each technique, and how to choose and employ the techniques when designing their study.
The course includes practical work with hands-on wet-lab work and imaging sessions as well as primary data analysis for three technologies in two practical formats: Visium/Spatial Transcriptomics (ST) as an unbiased method, and In Situ Sequencing (ISS)/Scrinshot as targeted methods.
The lectures will cover the following topics: Visium/ST, ISS/Scrinshot, osmFISH, seqFISH+, Slide-seqV2, MERFISH, CellPhoneDB as well as Human Cell Atlas.
The course is aimed at the graduate student and postdoctoral level, but applications from scientists at all levels are welcome.
Registration deadline: 18 March
joakim.lundeberg@scilifelab.se
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