[Spotlight seminar]- Ralf Jungmann
June 25, 2024 @ 15:00 – 16:00 CEST
Ralf Jungmann,
Professor at Ludwig-Maximilians-Universisty (LMU) and Max-Planck-Institut for Biochemistry, Germany
Seminar: Localizomics: towards spatial omics using DNA-based super-resolution microscopy
I will discuss our group’s vision of converting standard off-the-shelf fluorescence microscopy hardware into a tool for spatial omics. I will introduce technical advancements in DNA-PAINT including approaches that achieve sub-nanometer spatial resolution and spectrally unlimited multiplexing in whole cells. I will then discuss protein labeling probes such as Slow Off-rate Modified Aptamers (SOMAmers) that could allow DNA-barcoded labeling of much of the proteome in a cellular environment. Next, I present approaches to increase DNA-PAINT’s traditionally slow imaging speeds to achieve throughputs necessary for network-wide molecular interrogation. Finally, I will present cell surface receptor quantification at thus far elusive resolutions and levels of multiplexing.
Bio
Ralf Jungmann’s interdisciplinary research is aimed at developing novel imaging tools for biological and biomedical applications. Specifically, structural and dynamic DNA nanotechnology are combined with single-molecule fluorescence methods. The novel imaging tools unravel position and interplay of genes, RNAs and proteins in cells in a truly quantitative way with ultrahigh resolving power.
Read more: https://www.biochem.mpg.de/jungmann
Host: Hans Blom, KTH, hblom@kth.se
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