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Super-Resolution, Light-Sheet, STED-FCS and FRET-FCS

May 31, 2021 - June 11, 2021
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Super-Resolution, Light-Sheet, STED-FCS and FRET-FCS

May 31 @ 08:00 June 11 @ 17:00 CEST

The Advanced Light Microscopy Unit, ALM, at Scilifelab gives a two-week course focusing on four new imaging and fluorescence spectroscopy techniques:

Light-Sheet Imaging

  • Long time-lapse imaging of live model organisms with low phototoxicity
  • Ultrafast volumetric imaging of cells with lattice light-sheet microscopy

Super-Resolution Imaging in Living Cells – MoNaLISA

  • Smart probes. 50 nm resolution at 1-2 Hz. 
  • 100 x 100 mm field of view. Time lapse imaging

STED-FCS

  • Fluctuation spectroscopy with 30 nm resolution
  • Sub-diffraction dynamics in living cells

FRET-FCS

  • Easy detection of protein-protein interactions in living cells
  • Highly sensitive detection of rare oligomers in living cells

Lecturers

  • Steven Edwards
  • Erdinc Sezgin
  • Ilaria Testa
  • Stefan Wennmalm

Credits: 3 hp for PhD students, but open for anyone

Location: Web-based

Registration: stewen@kth.se