TEM sample preparation practical course part II
November 14 @ 08:00 – November 18 @ 17:00 CET
TEM sample preparation course – Chemical fixation, high pressure freezing, plastic embedding and staining methods including practice on ultra-microtome sectioning, TEM imaging and an introduction to CLEM (Correlative Light and Electron Microscopy), FIB-SEM (Focused Ion Beam) and electron tomography.
Pre-requisite criterion: Basic EM course at UCEM or equal previous experiences of EM methods.
Participants will practice EM sample preparation and EM imaging with one sample from their own project. Our lab space limits the number of participants to 8 so we will select participants based on your motivation and how your project aims fits to the course content. Please write a short project description, a motivation for participating and what sample you would like to process during the course.
Please, look at the program and the methods that are covered in this course. Make sure you have time to participate full days and the entire week. Since the fixation and plastic embedding takes time, you should bring one test-sample to Agnieszka on Monday 7 of November, and a new test-sample on the first course day, 14 November.
The course preliminary program will be announced here once it is organised.
Lectures at KBC and laboratory demonstrations at UCEM – Level 5, 6 + new EM building (Level 1), Umeå University.
8 persons. Open to all research students, postdocs and staff.
- Sara Henriksson – EM research engineer
- Gayathri Vegesna – EM research engineer
- Agnieszka Ziolkowska – EM research engineer
- Camilla Holmlund – EM research engineer
- Linda Sandblad – EM facility director
Coffee, cookies and fruit will be served.
No course fee, but full attendance is required!
Students that attend all theoretical and practical parts of the course, will receive a certificate for 1,5 hp.