TEM sample preparation practical course
April 22, 2024 @ 08:00 – April 26, 2024 @ 17:00 CEST
This is a practical course on sample preparation for TEM and includes:
- Ultra-microtome sectioning
- Chemical fixation
- Plastic embedding
- TEM imaging
- High-pressure freezing & freeze substitution
- Immunolabeling techniques (Immunogold, CLEM)
- Volume EM (FIB-SEM & Tomography)
Participants will practice TEM sample preparation and TEM imaging with a sample from their own project. Lab space limits the number of participants to 8, chosen by motivation and project aim. Write a short project description, motivation for participating and what sample you would like to bring for the course. Fixation and plastic embedding take time, therefore one test-sample should be brought to be prepared before the course, and a new test-sample is brought for the first day.
In addition, a small presentation about an EM-project idea of your own should be prepared and presented on the last day.
Participants should attend all theoretical and practical parts of the course to receive a certificate of 1,5 hp.
Pre-requisite criterion: Basic EM course at UCEM or previous equal experiences with EM methods.
Teachers: Sara Henriksson, Agnieszka Ziolkowska, Narin Mohamad and Linda Sandblad
Contact: linda.sandblad@umu.se
Application deadline: 27 March 2024.
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