The Cryo-EM Swedish National Facility offers access to state-of-the-art equipment and expertise in single particle cryo-EM and cryo-tomography (cryo-ET). The Facility has two nodes: at SciLifeLab in Stockholm and at Umeå Core Facility for Electron Microscopy at Umeå University. Our aim is to provide:
The Facility is supported by SciLifeLab, the Knut and Alice Wallenberg Foundation, the Family Erling Persson Foundation, the Kempe Foundations, Stockholm University and Umeå University.
The facility provides service and user training for all aspects of cryo-EM projects, including project planning, sample preparation, screening, data collection and data analysis.
SciLifeLab in Stockholm offers single-particle cryo-EM and cryo-ET of plunge-frozen samples, using a Talos Arctica for sample optimization/data acquisition and two Titan Krioses for high-resolution data collection. Development of a service for micro-ED is under way and diffraction projects are accepted. The Stockholm node offers also a bi-weekly drop-in service for help with image processing.
The Umeå node offers single-particle cryo-EM and cryo-ET of plunge-frozen samples with a Titan Krios, Focused Ion Beam (FIB) lamellae preparation for cryo-ET with a Scios DualBeam SEM and sample optimization support with a Talos L120. The Umeå node also offers a number of other TEM and SEM applications and sample preparation support, e.g. high pressure freezing, cryo-CLEM, for which further information can be found at www.umu.se/en/ucem.
We offer a biweekly Drop-In Service to help you with data processing and/or to discuss your to-be EM projects. We are available for counselling either in person of via Skype/Zoom. Just contact us at cryoEM_stockholm_lab@scilifelab.se or book one-hour slot in this calendar.
Using the Application Portal, the facilities can be accessed by Swedish researchers through a peer-reviewed routine. Two types of applications are available: Rapid Access (RA) and BAG (Block Allocation Group). RA applications can be submitted at any time, they are evaluated for technical feasibility and allocated time in the next quarter. BAG applications are evaluated once a year by a national Project Evaluation Committee, based on their scientific merit and technical feasibility.
Titan Krios G2, equipped with a Ceta CMOS, a Falcon III and Gatan K3 BioQuantum electron detectors and a Volta phase plate
Titan Krios G3i, equipped with a Ceta-D camera and a Gatan K3 BioQuantum detector
Talos Arctica equipped with Falcon III, Gatan K2 and Ceta CMOS with EPU-D for diffraction
Titan Krios G2, equipped with a Ceta CMOS, Falcon III and Gatan K2 BioQuantum electron detectors and Volta phase plate
Talos L120C, equipped with Ceta CMOS detectorScios DualBeam FIB-SEM, equipped with Trinity T1/T2, ET-SE, ICE, DBS, CCD, Nav-Cam detectors
Mailing Address
SciLifeLab
Cryo-EM, Gamma 2
Box 1031
171 21 Solna
Sweden
Visiting Address/Deliveries
SciLifeLab
Cryo-EM, Gamma2
Tomtebodav 23A
171 65 Solna
Sweden
Mailing Address
Umeå Core Facility for Electron Microscopy (UCEM)
Department of Chemistry
Umeå University
907 87 Umeå, Sweden
Visiting Address/Deliveries
Umeå Core Facility for Electron Microscopy (UCEM)
Chemical Biological Centre (KBC) Building
Umeå University
Linnaeus väg 6
907 36 Umeå, Sweden
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