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Swedish NMR Centre

National facility

The Swedish NMR Centre is an international, national and local research infrastructure that provides access to state-of-the-art NMR instrumentation and methodology, specifically within

  • Structural biology
  • Metabolomics
  • Chemical biology and small molecule NMR
  • Diffusion, microimaging and MAS NMR

Swedish NMR Centre comprises two nodes, in Gothenburg and Umeå, with complementary competences. The Gothenburg node has much focus on structural biology and metabolomics studies on liquids, whereas Umeå can cater for solid-state NMR metabolomics studies. Both nodes have capability for fragment-based screening. For more information, please visit us at Gothenburg or Umeå.

EQUIPMENT

NMR spectrometers and main probe configuration


Gothenburg

  • Oxford 900 pumped magnet, Bruker Avance III HD spectrometer, 5 mm TCI cryoprobe
  • Oxford 800 pumped magnet, Bruker Avance III HD spectrometer, cooled SampleJet sample changer, 3 mm TCI cryoprobe
  • Oxford 800 magnet,  Bruker Avance III HD spectrometer, SampleJet sample changer, 5 mm TXO cryoprobe
  • Bruker 700 Avance III HD spectrometer, SampleJet sample changer, 5 mm QCI cryoprobe
  • Varian INOVA 600 spectrometer, 3.2 mm MAS solids probe
  • Bruker 600 Avance III HD spectrometer, cooled SampleJet sample changer, alternately a 5 mm RT BBI probe for Bruker IVDr operation or a 5 mm diffusion/imaging probe

Umeå

  • Bruker 850 Avance III HD spectrometer, 5mm TXI cryoprobe, sample changer
  • Bruker 600 MHz Avance III, cooled SampleJet sample changer
  • Bruker 500 MHz Avance III

 

Sample preparation equipment


Gothenburg

  • Bruker SamplePro L liquid handling robot
  • Agilent Bravo liquid handling robot

Umeå

  • Bruker SamplePro L liquid handling robot

 

RECENT PROJECTS

Structural biology

  • Burmann BM, Gerez JA, Matecko-Burmann I, Campioni S, Kumari P, Ghosh D, Mazur A, Aspholm EE, Sulskis D, Wawrzyniuk M, et al. Regulation of alpha-synuclein by chaperones in mammalian cells. Nature 2019, doi:10.1038/s41586-019-1808-9.
  • Gustavsson E, Isaksson L, Persson C, Mayzel M, Brath U, Vrhovac L, Ihalainen JA, Karlsson BG, Orekhov V, Westenhoff S. Modulation of Structural Heterogeneity Controls Phytochrome Photoswitching. Biophysical Journal 2019, doi:https://doi.org/10.1016/j.bpj.2019.11.025.
  • Verteramo ML, Stenström O, Ignjatović MM, Caldararu O, Olsson MA, Manzoni F, Leffler H, Oksanen E, Logan DT, Nilsson UJ, et al. Interplay between Conformational Entropy and Solvation Entropy in Protein-Ligand Binding. Journal of the American Chemical Society 2019, 141:2012-2026, doi:10.1021/jacs.8b11099.
  • Zhou S, Pettersson P, Brzezinski P, Ädelroth P, Mäler L. NMR Study of Rcf2 Reveals an Unusual Dimeric Topology in Detergent Micelles. ChemBioChem 2018, 19:444-447, doi:10.1002/cbic.201700664.

Metabolomics

  • Lindqvist HM, Rådjursöga M, Malmodin D, Winkvist A, Ellegård L. Serum metabolite profiles of habitual diet: evaluation by 1H-nuclear magnetic resonance analysis. American Journal of Clinical Nutrition 2019, 110:53-62, doi:10.1093/ajcn/nqz032.
  • Malmodin D, Pedersen A, Karlsson BG, Forsander G. NMR Spectroscopic Analysis to Evaluate the Quality of Insulin: Concentration, Variability, and Excipient Content. J Diabetes Sci Technol 2019:1932296819831995, doi:10.1177/1932296819831995.
  • Andersson-Hall U, Gustavsson C, Pedersen A, Malmodin D, Joelsson L, Holmäng A. Higher concentrations of BCAAs and 3-HIB are associated with insulin resistance in the transition from gestational diabetes to type 2 diabetes. Journal of Diabetes Research 2018, 2018, doi:10.1155/2018/4207067.
  • Brunius C, Pedersen A, Malmodin D, Karlsson BG, Andersson LI, Tybring G, Landberg R. Prediction and modeling of pre-analytical sampling errors as a strategy to improve plasma NMR metabolomics data. Bioinformatics 2017, 33, doi:10.1093/bioinformatics/btx442.

Chemical biology

  • Michel, M., T. Visnes, E. J. Homan, B. Seashore-Ludlow, M. Hedenstrom, E. Wiita, K. Vallin, C. B. J. Paulin, J. X. Zhang, O. Wallner, M. Scobie, A. Schmidt, A. Jenmalm-Jensen, U. W. Berglund, and T. Helleday. Computational and Experimental Druggability Assessment of Human DNA Glycosylases, Acs Omega, 2019. 4: 11642-56

Data sampling, signal processing and data analysis

  • Qu X, Huang Y, Lu H, Qiu T, Guo D, Agback T, Orekhov V, Chen Z. Accelerated Nuclear Magnetic Resonance Spectroscopy with Deep Learning. Angew Chem Int Ed Engl 2019, doi:10.1002/anie.201908162.
  • Pustovalova Y, Mayzel M, Orekhov VY. XLSY: Extra-Large NMR Spectroscopy. Angew Chem Int Ed Engl 2018, 130:14239-14241, doi:10.1002/ange.201806144.