Björn Önfelt

KTH / KI

onfelt@kth.se

Keywords

Bioimaging, Cancer, Cytotoxic T cell, Immunology, Immunotherapy, Infectious disease, Nanotechnology, Natural killer cell, Single cell, Transplantation

Key Publications

I. Prager, C. Liesche, H. van Ooijen, D. Urlaub, Q Verron, N Sandström, F. Fasbender, M. Claus, R. Eils, J. Beaudouin, B. Önfelt, C. Watzl (2019) Natural Killer cells switch from Granzyme B to death receptor-mediated cytotoxicity during serial killing of tumor cells. Journal of Experimental Medicine 216(9):2113-2127. (https://pubmed.ncbi.nlm.nih.gov/31270246/)

Steven J. Edwards, Valentina Carannante, Kyra Kuhnigk, Henrik Ring, Tatsiana Tararuk, Finn Hallböök, Hans Blom, Björn Önfelt, Hjalmar Brismar (2020) High-resolution imaging of tumour spheroids and organoids enabled by expansion microscopy. Frontiers in Molecular Biosciences 7:208 (https://www.frontiersin.org/articles/10.3389/fmolb.2020.00208/full)

Quentin Verron, Elin Forslund, Ludwig Brandt, Mattias Leino, Thomas W. Frisk, Per E. Olofsson and Björn Önfelt (2021) NK cells integrate signals when building immune synapses but require local stimuli for degranulation, Science Signaling 14(684):eabe2740 (https://pubmed.ncbi.nlm.nih.gov/34035142/)

Niklas Sandström, Ludwig Brandt, Patrick Alain Sandoz, Chiara Zambarda, Karolin Guldevall, Malte Schulz-Ruhtenberg, Bernd Rösener, Robin Alexander Krüger, Björn Önfelt (2022) Live single cell imaging assays in glass microwells produced by laser-induced deep etching. Lab on a chip, 22, 2107-2121 (https://pubs.rsc.org/en/content/articlelanding/2022/lc/d2lc00090c)

Lucas C.M. Arruda, Arwen Stikvoort, Melanie Lambert, Liqing Jin, Laura Sanchez Rivera, Renato M.P. Alves, Tales Rocha De Moura, Carsten Mim, Sören Lehmann, Rebecca Axelsson-Robertson, John E. Dick, Jonas Mattsson, Björn Önfelt, Mattias Carlsten, Michael Uhlin (2022) A novel CD34-specific T-cell engager efficiently depletes acute myeloid leukemia and leukemic stem cells in vitro and in vivo. Haematologica 107(8):1786-1795 (https://pubmed.ncbi.nlm.nih.gov/35142149/)

Niklas Sandström*, Valentina Carannante*, Karl Olofsson, Patrick A. Sandoz, Elisabeth Moussaud-Lamodière, Brinton Seashore-Ludlow, Hanna Van Ooijen, Quentin Verron, Thomas Frisk, Madoka Takai, Martin Wiklund, Päivi Östling and Björn Önfelt (2022) Miniaturized and multiplexed high-content screening of drug and immune sensitivity in a novel multichambered microwell chip, Cell Reports Methods 2(7), 100256 (https://pubmed.ncbi.nlm.nih.gov/35880015/)

SciLifeLab / Contact / Björn Önfelt

Björn Önfelt

Research interests

The groups research is focused on studying mechanisms of immune surveillance of natural killer cells and gamma,delta T cells. For this we use combinations of microsystems technology and optical microscopy. We have developed a microchip platform that makes it possible to study the behavior and response of NK and T cells at the single cell level. With this we have, e.g. resolved heterogeneity in response within populations of human NK cells, identified NK cells with extraordinary potential to kill tumor cells (serial killers), investigated how NK cells utilize degranulation and death ligands for cytotoxicity, etc.

Group members

Björn Önfelt, Professor, PI
Karolin Guldevall, Researcher
Niklas Sandström, Postdoc
Patrick Sandoz, Postdoc
Laura Sanchez Rivera, Postdoc
Rento Alves, Postdoc
Ludwig Brandt, PhD-student
Quentin Verron, PhD-student
Valentina Carannante, PhD-student
Hanna van Ooijen , PhD-student
Karl Olofsson, PhD-student (cosupervisor)

Contact

bjorn.onfelt@scilifelab.se