Davide Angeletti

Key publications

Single cell BCR and RNA analysis after respiratory virus infection reveals spatiotemporal dynamics of antigen specific B cell response
Mathew NR, Jayanthan JK, Smirnov IV, Robinson JL, Axelsson H, Nakka SS, Emmanouilidi A, Czarnewski P, Yewdell WT, Schön K, Lebrero-Fernandéz C, Bernasconi V, Rodin W, Harandi AM, Lycke NY, Borcherding N, Yewdell JW, Greiff V, Bemark M, Angeletti D (2021)
Cell Reports. 35(12):109286

Longitudinal single-cell analysis of SARS-CoV-2-reactive B cells uncovers persistence of early-formed, antigen-specific clones
Scharf L*, Axelsson H*, Emmanouilidi A*, Mathew NR, Sheward DJ, Leach S, Isakson P, Smirnov IV, Marklund E, Miron N, Andersson LM, Gisslén M, Murrell B, Lundgren A, Bemark M, Angeletti D (2023)
JCI Insight. 2023;8(1):e165299

Defining B cell Immunodominance to Viruses
Angeletti D, Gibbs JS, Angel M, Kosik I, Hickman HD, Frank GM, Das S, Wheatley AK, Prabhakaran M, Leggat DJ, McDermott AB, Yewdell JW (2017)
Nature Immunology. 18(4), 456-463

Memory B cell diversity: from early generation to tissue residency and reactivation
Reusch L and Angeletti D (2023).
European Journal of Immunology. e2250085

Outflanking Immunodominance to Target Subdominant Broadly Neutralizing Epitopes
Angeletti D, Kosik I, Yewdell WT, Boudreau C, Mallajosyula VVA, Hickman HD, Chambers M, Prebhakaran M, McDermott AB, Alter G, Chaudhuri J, Yewdell JW (2019)
Proceedings of the National Academy of Science of the USA, 116(27):13474-13479

Seasonal influenza causes significant morbidity and mortality every year, with the possibility of global pandemic outbreaks. Current vaccines provide reasonable protection against circulating viruses but require yearly reformulation. Therefore, there is an urgent need to develop a vaccine that could provide long term immunity to the virus. Our work aims at defining the basic immunological mechanisms of antibody and B cell responses as an essential step towards an effective universal vaccination.

In peripheral lymphnodes, structures called germinal centers emerge after a challenge by a foreign antigen. These structures are primarily composed of B cells that bound with sufficient avidity to the antigen. In the germinal centers, B cells proliferate, increase their avidity for the antigen and finally differentiate into antibody secreting plasma cells.

But not all B cells are made equal: there are major differences between B cells of different specificities, even for distinct parts of the same molecule. Which B cells can enter the germinal center? which can proliferate? and which can differentiate? These phenomena are regulated by immunodominance. Immunodominance is the phenomenon of unequal immunogenicity between competing antigens or epitopes on the same antigen. In the group we use influenza A virus surface protein, hemagglutinin, as a model to gain a more complete understanding of the rules that govern immunodominance, and ways to manipulate it.

Projects in our group focus on different aspects of immunodominance and are aimed at defining its basic mechanisms in order to manipulate immunodominance and direct antibody responses towards conserved epitopes. We combine in vivo and in vitro studies, using a range of immunological techniques, multicolor flow cytometry and single cell sequencing in order to address these questions.

Group members

Davide Angeletti, PI
Nimitha R. Mathew, postdoc
Hsiang-Chi Huang, postdoc
Josue Enriquez, postdoc
Hannes Axelsson, PhD student
Laura Reusch, PhD student
Janarthan R. Murthi, PhD student
Danica F. Besavilla, PhD student
Romain Gailleton, PhD student
Zhaoshan Chen, visitng PhD student
Karin Schön, BMA

Last updated: 2023-04-04

Content Responsible: Hampus Persson(hampus.persson@scilifelab.uu.se)